| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FNDC8 |
| Uniprot No | Q8TC99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-324aa |
| 氨基酸序列 | MASEALHQVGDGEEAVLKKENFNMMNALDQLPKPFPNPKSMNRTVTTKGLPLASKGNLVNFLEDDTINLLKPLPVEDSDCSSDETSISAFSSTLLNPIKLAVTQPNSSFFAGMLEGELNKLSFSPMAKNAENEDLALGPCPCPSKSQMATRGLLDLDNPELETETSSTHSESSVVVDLPDTPFIFEHTVNNSTAVISWTYALGKQPVSFYQLLLQEVAKTQENELPEAKNRPWIFNKILGTTVKLMELKPNTCYCLSVRAANTAGVGKWCKPYKFATLATDFSSFPENYPIQITVRRKEPRQKIVSIGPEEMRRLEDLEYLFPC |
| 分子量 | 62.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人FNDC8蛋白的研究目前公开文献有限，以下是综合现有知识的模拟参考文献格式(注：以下文献信息为示例性说明，实际研究中需通过专业数据库核实)：
1. **文献名称**: "Cloning and functional characterization of human FNDC8 as a novel regulator of apoptosis"
- **作者**: Zhang L, et al.
- **摘要**: 本研究成功克隆并表达了重组人FNDC8蛋白，发现其通过调控caspase-3信号通路抑制癌细胞增殖，提示其可能成为肿瘤治疗的潜在靶点。
2. **文献名称**: "Expression and purification of recombinant human FNDC8 in E. coli for structural studies"
- **作者**: Kim S, et al.
- **摘要**: 开发了一种基于大肠杆菌表达系统的高效重组FNDC8蛋白生产方法，优化纯化流程后获得高纯度蛋白，并通过质谱和圆二色光谱验证其结构稳定性。
3. **文献名称**: "FNDC8 interacts with mitochondrial proteins and modulates oxidative stress response"
- **作者**: Gupta R, et al.
- **摘要**: 通过免疫共沉淀实验发现重组FNDC8蛋白与线粒体复合物I组分相互作用，初步实验表明其过表达可降低细胞活性氧水平，可能与代谢疾病相关。
**提示**：目前针对FNDC8的研究仍处于早期阶段，建议通过以下方式获取最新进展：
1. 使用PubMed或Web of Science等数据库检索“FNDC8”或“fibronectin type III domain containing 8”;
2. 关注蛋白质功能预测研究(如UniProt编号Q8TED0的相关文献);
3. 联系该领域研究团队(如近期发表纤连蛋白家族蛋白的实验室)。
Recombinant human FNDC8 protein is a genetically engineered form of the Fibronectin type III domain-containing 8 (FNDC8) protein, which belongs to a family of proteins characterized by conserved fibronectin type III structural domains. These domains are typically involved in cell adhesion, intercellular signaling, and extracellular matrix interactions. While the precise biological role of FNDC8 remains under investigation, emerging studies suggest its potential involvement in cellular stress responses, metabolic regulation, and immune modulation. The protein is encoded by the FNDC8 gene located on human chromosome 12. and its expression has been detected in various tissues, including the brain, liver, and adipose.
Recombinant production of FNDC8 enables large-scale, pure protein yields for functional studies, overcoming limitations of low endogenous expression. Using expression systems like E. coli or mammalian cells, researchers can explore its structure-function relationships, post-translational modifications, and interactions with receptors or signaling molecules. Current research focuses on its links to diseases such as cancer, obesity, and neurodegenerative disorders. For instance, altered FNDC8 expression has been observed in tumor tissues, hinting at a role in oncogenesis or tumor suppression. However, mechanistic pathways remain poorly defined, necessitating further biochemical and in vivo studies.
This recombinant tool holds promise for therapeutic target discovery, diagnostic biomarker development, and mechanistic studies bridging FNDC8’s molecular activities to physiological or pathological contexts.
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