| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXD4L6 |
| Uniprot No | Q3SYB3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417aa |
| 氨基酸序列 | MNLPRAERLR STPQRSLRDS DGEDGKIDVL GEEEDEDEVE DEEEAASQQF LEQSLQPGLQ VARWGGVALP REHIEGGGGP SDPSEFGTKF RAPPRSAAAS EDARQPAKPP YSYIALITMA ILQNPHKRLT LSGICAFISG RFPYYRRKFP AWQNSIRHNL SLNDCFVKIP REPGHPGKGN YWSLDPASQD MFDNGSFLRR RKRFKRHQLT PGAHLPHPFP LPAAHAALHN PHPGPLLGAP APPQPVPGAY PNTAPGRRPY ALLHPHPLRY LLLSAPVYAG APKKAEGAAL ATPAPFPCCS PHLVLSLGRR ARVWRRHREA DASLSALRVL CKGSGERVQG LRRVCPRPRG ATATCSSDHQ ACCIPRPLPL CCKCPPPPLL GQFCSNSSSI RRRTAPTAAL PPRARCWAGT CRPRRPC |
| 分子量 | 45.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FOXD4L6蛋白的示例参考文献(注：以下内容为假设性示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Cloning and Functional Characterization of Recombinant Human FOXD4L6 Protein"*
**作者**: Zhang Y, et al.
**摘要**: 本研究成功克隆了人源FOXD4L6基因，并在哺乳动物细胞中表达纯化了重组蛋白。功能实验表明，FOXD4L6通过结合特定DNA序列调控胚胎干细胞的分化。
2. **文献名称**: *"FOXD4L6: A Novel Regulator of Neural Crest Development"*
**作者**: Lee S, Smith J.
**摘要**: 报道了重组FOXD4L6蛋白在斑马鱼模型中的功能研究，证明其通过激活Wnt/β-catenin通路促进神经嵴细胞的迁移和分化。
3. **文献名称**: *"Structural Insights into the DNA-Binding Mechanism of FOXD4L6"*
**作者**: Gupta R, et al.
**摘要**: 利用X射线晶体学解析了重组FOXD4L6蛋白的DNA结合域结构，揭示了其与其他FOX家族成员不同的独特结合位点。
4. **文献名称**: *"High-Yield Production of Recombinant FOXD4L6 in a Bacterial System"*
**作者**: Wang H, et al.
**摘要**: 开发了一种优化的大肠杆菌表达系统，用于高效生产可溶性重组FOXD4L6蛋白，并验证了其体外生物活性。
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**注意**：以上文献为虚拟示例，实际研究中请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 搜索真实文献，建议使用关键词 "recombinant FOXD4L6" 或 "FOXD4L6 protein function" 进行检索。若确无相关文献，可能需确认蛋白名称准确性(如是否更名为FOXD4或与FOX家族其他成员相关)。
Recombinant human FOXD4L6 protein is a genetically engineered version of the FOXD4L6 transcription factor, belonging to the Forkhead box (FOX) protein family. FOX proteins are characterized by a conserved "forkhead" or "winged-helix" DNA-binding domain, which enables sequence-specific interactions with target genes to regulate cellular processes like development, differentiation, and metabolism. The FOXD4 subfamily, including FOXD4L6. shares structural homology but exhibits functional diversity. While FOXD4L6 remains less studied compared to its paralogs (e.g., FOXD3. a pluripotency regulator in stem cells), emerging evidence suggests its involvement in early embryonic development and neural differentiation.
The recombinant protein is typically produced in bacterial or mammalian expression systems for *in vitro* studies. Its availability facilitates exploration of FOXD4L6’s molecular mechanisms, such as DNA-binding specificity, transcriptional activation/repression roles, and interactions with co-regulators. Research implicates FOXD4-like proteins in maintaining progenitor cell states and modulating signaling pathways (e.g., Wnt/β-catenin). Dysregulation of FOX transcription factors is often linked to developmental disorders and cancers, though FOXD4L6’s direct pathological relevance requires further validation.
Current applications include using recombinant FOXD4L6 in chromatin immunoprecipitation (ChIP), gene editing assays, and disease modeling. Its potential therapeutic relevance lies in regenerative medicine or targeting transcriptionally dysregulated malignancies, pending functional characterization.
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