| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOSB |
| Uniprot No | P53539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338aa |
| 氨基酸序列 | MFQAFPGDYDSGSRCSSSPSAESQYLSSVDSFGSPPTAAASQECAGLGEMPGSFVPTVTAITTSQDLQWLVQPTLISSMAQSQGQPLASQPPVVDPYDMPGTSYSTPGMSGYSSGGASGSGGPSTSGTTSGPGPARPARARPRRPREETLTPEEEEKRRVRRERNKLAAAKCRNRRRELTDRLQAETDQLEEEKAELESEIAELQKEKERLEFVLVAHKPGCKIPYEEGPGPGPLAEVRDLPGSAPAKEDGFSWLLPPPPPPPLPFQTSQDAPPNLTASLFTHSEVQVLGDPFPVVNPSYTSSFVLTCPEVSAFAGAQRTSGSDQPSDPLNSPSLLAL |
| 分子量 | 40.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FOSB蛋白的模拟参考文献示例(注：文献为虚构，仅供格式参考)：
1. **《重组人FOSB蛋白的克隆表达及功能初探》**
作者：Zhang L, Wang Y, et al.
摘要：研究成功构建了重组人FOSB蛋白的原核表达系统，通过大肠杆菌表达并纯化获得高纯度蛋白。实验验证其与JUN蛋白的结合能力，提示其在AP-1转录复合物中的潜在调控作用。
2. **《FOSB蛋白在神经可塑性中的分子机制》**
作者：Chen R, Nestler EJ.
摘要：通过重组FOSB蛋白体外实验，发现其剪接变体ΔFosB在小鼠成瘾模型中长期表达，调控下游靶基因如BDNF，揭示了其在神经适应性及行为成瘾中的关键角色。
3. **《重组FOSB磷酸化修饰对肿瘤细胞增殖的影响》**
作者：Kim S, Lee JH.
摘要：利用昆虫细胞系统表达重组人FOSB蛋白，分析其磷酸化修饰位点。体外实验表明，磷酸化FOSB通过MAPK通路促进乳腺癌细胞增殖，为癌症靶向治疗提供新思路。
4. **《FOSB/JUN复合物的结构解析及其DNA结合特性》**
作者：Smith T, García A.
摘要：通过X射线晶体学解析重组FOSB-JUN异源二聚体结构，揭示其与DNA结合域的关键氨基酸残基，为设计干预AP-1活性的小分子药物奠定结构基础。
(注：以上文献及内容为模拟虚构，实际引用请核实真实学术数据库。)
The FOSB protein, a member of the FOS family of transcription factors, plays critical roles in regulating gene expression by forming the AP-1 transcriptional complex with JUN proteins. It is involved in diverse cellular processes, including proliferation, differentiation, apoptosis, and response to stress or extracellular signals. Recombinant human FOSB protein is generated through molecular cloning and expression in heterologous systems (e.g., E. coli or mammalian cells) to study its structure, function, and interactions. Interest in FOSB stems from its association with neurological and psychological pathways, particularly its ΔFosB isoform, which accumulates in specific brain regions following chronic stimuli (e.g., drugs of abuse, stress) and mediates long-term neuroplasticity. Recombinant FOSB enables mechanistic studies of its role in addiction, depression, and neurodegenerative disorders. Additionally, it serves as a tool for investigating cancer progression, as aberrant AP-1 activity is linked to tumorigenesis and metastasis. The protein’s modular structure, including a basic leucine zipper (bZIP) domain for DNA binding and dimerization, is often studied using recombinant variants to dissect signaling pathways or screen therapeutic agents. Its applications span basic research, drug discovery, and biomarker development, underscoring its importance in both physiological and pathological contexts.
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