| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXD4L1 |
| Uniprot No | Q9NU39 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MNLPRAERPRSTPQRSLRDSDGEDGKIDVLGEEEDEDEVEDEEEEASQKFLEQSLQPGLQVARWGGVALPREHIEGGGPSDPSEFGTEFRAPPRSTAASEDARQPAKPPYSYIALITMAILQSPHKRLTLSGICAFISGRFPYYRRKFPAWQNSIRHNLSLNDCFVKIPREPGHPGKGTYWSLDPASQDMFDNGSFLRRRKRFKRHQLTPGAHLPHPFPLPAAHAALHNPRPGPLLGAPALPQPVPGAYPNTAPGRRPYALLHPHPPRYLLLSAPAYAGAPKKAEGADLATPGTLPVLQPSLGPQPWEEGKGLASPPGGGCISFSIESIMQGVRGAGTGAAQSLSPTAWSYCPLLQRPSSLSDNFAATAAASGGGLRQRLRSHQGRGAGRAPVGRVGAAAVSGGGRGL |
| 分子量 | 70 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人FOXD4L1蛋白的虚拟参考文献示例(注：实际文献可能需要通过学术数据库检索确认)：
1. **文献名称**：*"Expression and Functional Analysis of Recombinant Human FOXD4L1 in Stem Cell Maintenance"*
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌中成功表达重组人FOXD4L1蛋白，并证明其通过调控SOX2和OCT4基因维持胚胎干细胞的自我更新能力。
2. **文献名称**：*"FOXD4L1 Recombinant Protein Induces Neuronal Differentiation: A Role in Neurogenesis"*
**作者**：Wang X, Li M.
**摘要**：纯化的重组FOXD4L1蛋白在体外促进神经前体细胞分化为神经元，提示其在神经发育中的关键作用，机制可能与MAPK信号通路相关。
3. **文献名称**：*"Structural Characterization and Cancer-Associated Signaling of FOXD4L1 Recombinant Protein"*
**作者**：Garcia R, et al.
**摘要**：通过哺乳动物细胞系统表达重组FOXD4L1.解析其DNA结合结构域，并发现其在乳腺癌细胞中激活Wnt/β-catenin通路，促进肿瘤侵袭。
4. **文献名称**：*"Development of a FOXD4L1-Specific ELISA Using Recombinant Protein for Clinical Biomarker Detection"*
**作者**：Tanaka K, et al.
**摘要**：利用重组FOXD4L1蛋白开发高灵敏度ELISA检测法，验证其在结直肠癌患者血清中的高表达与转移风险的相关性。
如需实际文献，建议通过 **PubMed/Google Scholar** 检索关键词 *"Recombinant human FOXD4L1"* 或 *"FOXD4L1 protein function"*。
Recombinant human FOXD4L1 protein is a genetically engineered form of the forkhead box (FOX) family transcription factor, FOXD4L1 (Forkhead Box D4-Like 1), which plays a role in regulating gene expression during early embryonic development and cellular differentiation. FOX proteins are characterized by a conserved DNA-binding domain called the forkhead box or winged-helix domain, which enables sequence-specific interactions with target genes. FOXD4L1 is closely related to FOXD4. a protein implicated in neurogenesis, neural crest development, and maintenance of pluripotency in stem cells. While the precise biological functions of FOXD4L1 remain less characterized compared to other FOX family members, studies suggest its involvement in cellular reprogramming and tissue-specific gene regulation.
The recombinant form is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity for research applications. Researchers use recombinant FOXD4L1 to investigate its DNA-binding properties, transcriptional regulatory mechanisms, and potential roles in developmental disorders or cancers. Its overexpression or knockdown in cell models helps elucidate pathways linked to differentiation, proliferation, or apoptosis. Additionally, recombinant FOXD4L1 serves as a tool for studying epigenetic interactions, such as chromatin remodeling or partnerships with co-regulators. Despite limited clinical data, its study contributes to understanding early embryogenesis and may inform strategies in regenerative medicine or disease modeling. Optimization of its production often includes tags (e.g., His-tag) for affinity purification, followed by functional validation via electrophoretic mobility shift assays (EMSA) or luciferase reporter systems.
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