| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXJ3 |
| Uniprot No | Q9UPW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 氨基酸序列 | MGLYGQACPSVTSLRMTSELESSLTSMDWLPQLTMRAAIQKSDATQNAHGTGISKKNALLDPNTTLDQEEVQQHKDGKPPYSYASLITFAINSSPKKKMTLSEIYQWICDNFPYYREAGSGWKNSIRHNLSLNKCFLKVPRSKDDPGKGSYWAIDTNPKEDVLPTRPKKRARSVERASTPYSIDSDSLGMECIISGSASPTLAINTVTNKVTLYNTDQDGSDSPRSSLNNSLSDQSLASVNLNSVGSVHSYTPVTSHPESVSQSLTPQQQPQYNLPERDKQLLFSEYNFEDLSASFRSLYKSVFEQSLSQQGLMNIPSESSQQSHTSCTYQHSPSSTVSTHPHSNQSSLSNSHGSGLNTTGSNSVAQVSLSHPQMHTQPSPHPPHRPHGLPQHPQRSPHPAPHPQQHSQLQSPHPQHPSPHQHIQHHPNHQHQTLTHQAPPPPQQVSCNSGVSNDWYATLDMLKESCRIASSVNWSDVDLSQFQGLMESMRQADLKNWSLDQVQFADLCSSLNQFFTQTGLIHSQSNVQQNVCHGAMHPTKPSQHIGTGNLYIDSRQNLPPSVMPPPGYPHIPQALSTPGTTMAGHHRAMNQQHMMPSQAFQMRRSLPPDDIQDDFDWDSIV |
| 分子量 | 68.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为示例性内容，旨在展示可能的文献结构和研究方向，并非真实存在的论文。如需准确文献，请查阅学术数据库(如PubMed、Google Scholar)并使用关键词“recombinant human FOXJ3”或“FOXJ3 protein function”进行检索。
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1. **《Cloning and Expression of Recombinant Human FOXJ3 in a Mammalian System》**
**作者：** Zhang, L. et al.
**摘要：** 本研究报道了人源FOXJ3基因的克隆及其在哺乳动物细胞中的重组表达，成功纯化出高活性FOXJ3蛋白，并验证其与特定DNA序列的结合能力。
2. **《FOXJ3 Regulates Cell Cycle Progression through p53 Signaling Pathway》**
**作者：** Kim, S. & Patel, R.
**摘要：** 通过体外实验分析重组FOXJ3蛋白的功能，发现其通过调控p53相关通路影响细胞周期进程，提示其在肿瘤抑制中的潜在作用。
3. **《Structural and Functional Insights into Human FOXJ3 Protein》**
**作者：** Müller, T. et al.
**摘要：** 利用重组FOXJ3蛋白进行结晶学研究，解析了其三维结构，揭示了其与同类转录因子不同的DNA结合域特征。
4. **《FOXJ3 Involvement in Spermatogenesis: Evidence from Recombinant Protein Studies》**
**作者：** Gonzalez, E. et al.
**摘要：** 通过重组FOXJ3蛋白的体外功能实验，证明其在精子发生过程中参与调控生殖细胞特异性基因的表达。
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建议结合具体研究方向，在学术平台进一步检索最新文献以获取真实数据。
**Background of Recombinant Human FOXJ3 Protein**
FOXJ3. a member of the Forkhead box (FOX) transcription factor family, is characterized by a conserved forkhead DNA-binding domain. FOX proteins regulate diverse biological processes, including embryonic development, tissue homeostasis, and metabolism. FOXJ3. though less studied compared to other FOX members like FOXO or FOXP, has emerged as a critical player in cell cycle regulation, apoptosis, and gene expression control. It is implicated in modulating transcriptional programs associated with cellular proliferation and stress responses, with potential roles in cancer and stem cell biology.
Studies suggest FOXJ3 interacts with cell cycle regulators such as cyclin-dependent kinases (CDKs) and may suppress tumorigenesis by inducing cell cycle arrest. Its expression is tissue-specific, predominantly observed in testis, brain, and certain cancer cells, hinting at context-dependent functions. However, the molecular mechanisms underlying FOXJ3 activity remain unclear, partly due to limited availability of functional protein tools.
Recombinant human FOXJ3 protein, produced via *E. coli* or mammalian expression systems, enables *in vitro* studies to dissect its biochemical properties, DNA-binding specificity, and interaction networks. Purification tags (e.g., His-tag) facilitate isolation, while validated activity in EMSA or luciferase assays ensures reliability. This recombinant tool accelerates research into FOXJ3's role in development, disease, and potential therapeutic targeting, addressing gaps in FOX protein biology.
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