| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXN2 |
| Uniprot No | P32314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MGPVIGMTPDKRAETPGAEKIAGLSQIYKMGSLPEAVDAARPKATLVDSESADDELTNLNWLHESTNLLTNFSLGSEGLPIVSPLYDIEGDDVPSFGPACYQNPEKKSATSKPPYSFSLLIYMAIEHSPNKCLPVKEIYSWILDHFPYFATAPTGWKNSVRHNLSLNKCFQKVERSHGKVNGKGSLWCVDPEYKPNLIQALKKQPFSSASSQNGSLSPHYLSSVIKQNQVRNLKESDIDAAAAMMLLNTSIEQGILECEKPLPLKTALQKKRSYGNAFHHPSAVRLQESDSLATSIDPKEDHNYSASSMAAQRCASRSSVSSLSSVDEVYEFIPKNSHVGSDGSEGFHSEEDTDVDYEDDPLGDSGYASQPCAKISEKGQSGKKMRKQTCQEIDEELKEAAGSLLHLAGIRTCLGSLISTAKTQNQKQRKK |
| 分子量 | 47.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据现有知识整理的 **模拟参考文献示例**(仅供参考格式，非真实文献)：
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1. **文献名称**: "FOXN2 regulates invasion and metastasis in lung adenocarcinoma through TGF-β/Smad signaling"
**作者**: Wang, L., et al.
**摘要**: 研究重组人FOXN2蛋白在肺癌细胞中的功能，发现其过表达显著抑制TGF-β介导的EMT过程，并通过调控Smad蛋白磷酸化抑制肿瘤转移。
2. **文献名称**: "Recombinant human FOXN2 protein purification and its role in T-cell development"
**作者**: Zhang, Y., et al.
**摘要**: 报道利用大肠杆菌表达系统高效纯化重组FOXN2蛋白，并证实其通过与胸腺上皮细胞特定基因启动子结合，调控T细胞分化关键因子。
3. **文献名称**: "FOXN2 acts as a tumor suppressor in glioblastoma by modulating p53 pathway"
**作者**: Chen, H., et al.
**摘要**: 通过体外重组FOXN2蛋白功能实验，揭示其通过稳定p53蛋白并增强下游促凋亡基因转录，抑制胶质母细胞瘤增殖。
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**建议**：如需真实文献，可使用以下关键词在学术数据库中检索：
- "Recombinant human FOXN2 protein"
- "FOXN2 function in cancer"
- "FOXN2 transcriptional regulation"
推荐平台：PubMed (https://pubmed.ncbi.nlm.nih.gov) 或 Google Scholar。
The forkhead box (FOX) family of transcription factors plays critical roles in regulating gene expression during development, homeostasis, and disease. FOXN2. a member of the FOXN subfamily, is a nuclear protein encoded by the *FOXN2* gene located on human chromosome 2q34. It contains a highly conserved forkhead DNA-binding domain that enables sequence-specific interactions with target genes. FOXN2 is broadly expressed in tissues, particularly in the brain, thymus, and epithelial cells, where it participates in regulating cell differentiation, proliferation, apoptosis, and senescence.
Studies suggest FOXN2 functions as both a tumor suppressor and context-dependent oncogene. It modulates pathways like Wnt/β-catenin and p53. influencing cancer progression, neurodegeneration, and immune responses. Dysregulation of FOXN2 has been linked to gliomas, breast cancer, and age-related pathologies. Recombinant human FOXN2 protein, typically produced in *E. coli* or mammalian expression systems, retains post-translational modification capacity and biological activity. This engineered protein serves as a vital tool for *in vitro* studies, enabling mechanistic exploration of FOXN2’s role in chromatin remodeling, protein-protein interactions, and therapeutic targeting. Its application spans drug discovery, biomarker development, and gene therapy research, highlighting its dual significance in basic and translational biomedical research.
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