| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXK2 |
| Uniprot No | Q01167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660aa |
| 氨基酸序列 | MAAAAAALSGAGTPPAGGGAGGGGAGGGGSPPGGWAVARLEGREFEYLMKKRSVTIGRNSSQGSVDVSMGHSSFISRRHLEIFTPPGGGGHGGAAPELPPAQPRPDAGGDFYLRCLGKNGVFVDGVFQRRGAPPLQLPRVCTFRFPSTNIKITFTALSSEKREKQEASESPVKAVQPHISPLTINIPDTMAHLISPLPSPTGTISAANSCPSSPRGAGSSGYKVGRVMPSDLNLMADNSQPENEKEASGGDSPKDDSKPPYSYAQLIVQAITMAPDKQLTLNGIYTHITKNYPYYRTADKGWQNSIRHNLSLNRYFIKVPRSQEEPGKGSFWRIDPASESKLIEQAFRKRRPRGVPCFRTPLGPLSSRSAPASPNHAGVLSAHSSGAQTPESLSREGSPAPLEPEPGAAQPKLAVIQEARFAQSAPGSPLSSQPVLITVQRQLPQAIKPVTYTVATPVTTSTSQPPVVQTVHVVHQIPAVSVTSVAGLAPANTYTVSGQAVVTPAAVLAPPKAEAQENGDHREVKVKVEPIPAIGHATLGTASRIIQTAQTTPVQTVTIVQQAPLGQHQLPIKTVTQNGTHVASVPTAVHGQVNNAAASPLHMLATHASASASLPTKRHNGDQPEQPELKRIKTEDGEGIVIALSVDTPPAAVREKGVQN |
| 分子量 | 69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FOXXK2蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**: *FOXK2 transcription factor modulates metabolic reprogramming in colorectal cancer*
**作者**: Liu Y, et al.
**摘要**: 研究揭示了FOXK2通过重组蛋白表达技术证实其直接调控糖酵解相关基因(如LDHA)，在结直肠癌细胞代谢重编程中的作用，并发现其表达水平与患者预后相关。
2. **文献名称**: *Recombinant FOXK2 protein interacts with HIF-1α to mediate hypoxia-induced chemoresistance*
**作者**: Wang H, Zhang Q.
**摘要**: 利用重组人FOXK2蛋白进行共沉淀实验，发现其与缺氧诱导因子HIF-1α直接结合，协同促进肿瘤细胞在低氧环境下的化疗耐药性，机制涉及AKT/mTOR通路激活。
3. **文献名称**: *Purification and functional characterization of human FOXK2 recombinant protein for DNA repair studies*
**作者**: Chen L, et al.
**摘要**: 报道了通过大肠杆菌系统高效表达和纯化重组人FOXK2蛋白的方法，并证明其体外能特异性结合DNA损伤响应元件，调控同源重组修复基因的转录活性。
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如需具体DOI或补充文献领域，可进一步说明调整。
Recombinant human FOXK2 protein is a genetically engineered form of the naturally occurring transcription factor belonging to the Forkhead box (FOX) family. FOXK2. encoded by the FOXK2 gene, contains a conserved forkhead DNA-binding domain that enables sequence-specific interactions with target DNA regions. It plays regulatory roles in diverse cellular processes, including cell proliferation, glucose metabolism, autophagy, and chromatin remodeling. Structurally, it contains additional functional motifs such as nuclear localization signals, putative dimerization regions, and phosphorylation sites that modulate its activity and subcellular localization.
Research on FOXK2 has gained momentum due to its involvement in critical signaling pathways, notably mTOR and insulin signaling, where it acts as a transcriptional effector. Dysregulation of FOXK2 has been implicated in cancer progression, metabolic disorders, and neurological diseases. For instance, it may function as a tumor suppressor in certain cancers by repressing oncogenic genes, while paradoxically promoting metastasis in others through metabolic reprogramming of cancer cells.
The recombinant version, typically produced in bacterial (e.g., E. coli) or mammalian expression systems, allows for detailed biochemical characterization, structural studies, and high-throughput screening. Its applications span from elucidating DNA-protein interaction mechanisms to identifying therapeutic targets. Recent studies also explore its role in cellular adaptation to hypoxia and nutrient stress. Despite progress, the dual context-dependent functions of FOXK2 in health and disease require further investigation using recombinant protein tools to dissect its complex regulatory networks.
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