| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXO1A |
| Uniprot No | Q12778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-655aa |
| 氨基酸序列 | MAEAPQVVEI DPDFEPLPRP RSCTWPLPRP EFSQSNSATS SPAPSGSAAA NPDAAAGLPS ASAAAVSADF MSNLSLLEES EDFPQAPGSV AAAVAAAAAA AATGGLCGDF QGPEAGCLHP APPQPPPPGP LSQHPPVPPA AAGPLAGQPR KSSSSRRNAW GNLSYADLIT KAIESSAEKR LTLSQIYEWM VKSVPYFKDK GDSNSSAGWK NSIRHNLSLH SKFIRVQNEG TGKSSWWMLN PEGGKSGKSP RRRAASMDNN SKFAKSRSRA AKKKASLQSG QEGAGDSPGS QFSKWPASPG SHSNDDFDNW STFRPRTSSN ASTISGRLSP IMTEQDDLGE GDVHSMVYPP SAAKMASTLP SLSEISNPEN MENLLDNLNL LSSPTSLTVS TQSSPGTMMQ QTPCYSFAPP NTSLNSPSPN YQKYTYGQSS MSPLPQMPIQ TLQDNKSSYG GMSQYNCAPG LLKELLTSDS PPHNDIMTPV DPGVAQPNSR VLGQNVMMGP NSVMSTYGSQ ASHNKMMNPS SHTHPGHAQQ TSAVNGRPLP HTVSTMPHTS GMNRLTQVKT PVQVPLPHPM QMSALGGYSS VSSCNGYGRM GLLHQEKLPS DLDGMFIERL DCDMESIIRN DLMDGDTLDF NFDNVLPNQS FPHSVKTTTH SWVSG |
| 分子量 | 69.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为示例性参考文献条目(基于学术文献的常见研究主题构建，供参考)：
1. **文献名称**：*FOXO1A regulates apoptosis through transcriptional control of Bim in human cancer cells*
**作者**：Thompson, E. B., et al.
**摘要**：研究揭示了重组人FOXO1A蛋白通过直接结合Bim基因启动子调控细胞凋亡的分子机制，证明其在癌症细胞中的抑癌功能依赖于对Bim表达的激活。
2. **文献名称**：*Crystal structure of human FOXO1A DNA-binding domain complexed with a Forkhead response element*
**作者**：Tsai, K. L., et al.
**摘要**：报道了重组FOXO1A蛋白DNA结合域的高分辨率晶体结构，阐明了其与靶DNA序列相互作用的特异性位点，为设计靶向FOXO1的小分子药物提供结构基础。
3. **文献名称**：*Recombinant FOXO1A modulates hepatic gluconeogenesis in diabetic mouse models*
**作者**：Zhang, Y., & Accili, D.
**摘要**：通过体外表达重组FOXO1A蛋白，研究发现其通过抑制PGC-1α信号通路降低糖尿病模型小鼠肝脏糖异生，提示其作为2型糖尿病治疗靶点的潜力。
4. **文献名称**：*Post-translational modification analysis of recombinant human FOXO1A in oxidative stress response*
**作者**：Brunet, A., et al.
**摘要**：探讨了重组FOXO1A蛋白在氧化应激条件下的磷酸化、泛素化修饰模式变化，揭示AKT和SIRT1对其核质穿梭的协同调控机制。
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注：以上文献信息为示例性虚构内容，实际研究需以真实发表的学术文献为依据。建议通过PubMed、Web of Science等数据库以“recombinant human FOXO1A”为关键词检索最新论文。
Recombinant human FOXO1A protein is a lab-engineered version of the naturally occurring FOXO1A (Forkhead box class O1A) transcription factor, a key regulator in cellular homeostasis. FOXO1A belongs to the evolutionarily conserved FOXO family, which integrates signals from growth factors, nutrients, and stress to modulate gene expression. It plays pivotal roles in cell cycle arrest, apoptosis, oxidative stress resistance, glucose metabolism, and longevity. Under normal conditions, FOXO1A activity is tightly controlled by the PI3K/AKT signaling pathway. AKT phosphorylates FOXO1A, prompting its nuclear exclusion and cytoplasmic sequestration, thereby inhibiting its transcriptional functions. Dysregulation of FOXO1A is implicated in cancer, diabetes, aging-related disorders, and metabolic syndromes.
The recombinant form, typically produced in bacterial or mammalian expression systems, retains the functional domains of native FOXO1A, including the DNA-binding forkhead domain and regulatory phosphorylation sites. Its production enables in vitro and in vivo studies of FOXO1A-mediated signaling mechanisms, interactions with binding partners (e.g., SIRT1. β-catenin), and pharmacological modulation. Researchers employ recombinant FOXO1A to investigate insulin resistance pathways, tumor suppression mechanisms, and therapeutic interventions targeting FOXO-related diseases. Its availability accelerates drug screening and mechanistic insights, bridging molecular biology with translational medicine.
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