| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC43 |
| Uniprot No | Q96MW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAPSEV AAIAPGEGDG GGGGFGSWLD GRLEALGVDR AVYGAYILGI LQEEEEEEKL DALQGILSAF LEEDSLLNIC KEIVERWSET QNVVTKVKKE DEVQAIATLI EKQAQIVVKP RMVSEEEKQR KAALLAQYAD VTDEEDEADE KDDSGATTMN IGSDKLLFRN TNVEDVLNAR KLERDSLRDE SQRKKEQDKL QRERDKLAKQ ERKEKEKKRT QRGERKR |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于CCDC43重组蛋白的研究目前公开文献较少，以下是基于相关领域推测的潜在研究方向及假设性文献示例(仅供参考，建议通过学术数据库核实)：
1. **文献名称**：*Expression and Purification of Recombinant CCDC43 Protein in E. coli*
**作者**：Smith A, et al.
**摘要**：报道了在大肠杆菌中克隆并表达CCDC43重组蛋白的优化方法，通过亲和层析纯化，并验证了其结构稳定性，为后续功能研究奠定基础。
2. **文献名称**：*CCDC43 Interacts with Microtubule-Associated Proteins in Spermatogenesis*
**作者**：Zhang L, et al.
**摘要**：利用重组CCDC43蛋白进行免疫共沉淀实验，发现其与精子发生过程中的微管结合蛋白相互作用，提示其在生殖细胞发育中的潜在作用。
3. **文献名称**：*CCDC43 Overexpression Promotes Tumor Cell Invasion via ERK Signaling*
**作者**：Wang Y, et al.
**摘要**：通过体外表达重组CCDC43蛋白，发现其过表达激活ERK通路，增强癌细胞侵袭能力，提示其作为癌症治疗靶点的可能性。
**提示**：以上为推测性内容。建议使用PubMed、Web of Science等平台，以“CCDC43 recombinant”或“CCDC43 expression”为关键词检索最新文献，或关注该基因在生殖生物学、肿瘤学等领域的功能研究。
**Background of CCDC43 Recombinant Protein**
CCDC43 (Coiled-Coil Domain-Containing Protein 43) is a member of the coiled-coil domain-containing protein family, characterized by structural motifs that mediate protein-protein interactions and cellular scaffolding. While its exact biological functions remain under investigation, CCDC43 is hypothesized to play roles in cellular processes such as cytoskeletal organization, intracellular transport, or ciliary function, based on homology to related proteins. The gene encoding CCDC43 is conserved across vertebrates, suggesting evolutionary importance, though its expression patterns and regulation are not yet fully mapped.
Recombinant CCDC43 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional and structural studies. This involves cloning the CCDC43 gene into expression vectors, followed by purification via affinity tags (e.g., His-tag, GST-tag). Recombinant versions allow researchers to bypass challenges in isolating the native protein from tissues, ensuring higher yield and purity for experiments.
Current research focuses on elucidating CCDC43's interactions with other proteins or cellular components. Potential links to diseases, such as ciliopathies or cancer, are being explored, as coiled-coil proteins often contribute to cell cycle regulation or signaling pathways. However, direct evidence tying CCDC43 to specific pathologies remains limited.
The availability of recombinant CCDC43 accelerates antibody development, enzymatic assays, and structural analyses (e.g., X-ray crystallography, cryo-EM), which are critical for defining its molecular mechanisms. Further studies are needed to clarify its physiological roles and therapeutic relevance, positioning CCDC43 as an emerging target in cell biology and biomedical research.
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