| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMC1 |
| Uniprot No | P62191 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-440aa |
| 氨基酸序列 | GQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD DLRGTPMSVG TLEEIIDDNH AIVSTSVGSE HYVSILSFVD KDLLEPGCSV LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP QETYADIGGL DNQIQEIKES VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA NQTSATFLRV VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK IEFPLPDEKT KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT EAGLMALRER RMKVTNEDFK KSKENVLYKK QEGTPEGLYL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于PSMC1重组蛋白的参考文献及简要摘要：
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1. **文献名称**: *Structural insights into the functional cycle of the proteasome regulatory particle*
**作者**: Lander GC, et al.
**摘要**: 通过冷冻电镜技术解析了重组PSMC1蛋白在26S蛋白酶体调控复合体中的构象变化，揭示了其ATP酶活性在底物识别和去泛素化中的作用。
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2. **文献名称**: *PSMC1 variants in neurodegenerative diseases: Functional analysis of recombinant mutants*
**作者**: Wang Y, et al.
**摘要**: 利用重组PSMC1蛋白研究其基因突变对蛋白酶体功能的影响，发现特定突变导致ATP酶活性丧失，与早发性帕金森病相关。
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3. **文献名称**: *Reconstitution of human proteasome subunits reveals PSMC1's role in assembly*
**作者**: Smith J, Tanaka K.
**摘要**: 通过重组表达PSMC1与其他蛋白酶体亚基，证明PSMC1在调控复合体组装中的必要性，并阐明其与PSMC2的相互作用机制。
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4. **文献名称**: *Targeting PSMC1 in cancer therapy: Biochemical characterization of recombinant protein inhibitors*
**作者**: Chen L, et al.
**摘要**: 基于重组PSMC1蛋白筛选小分子抑制剂，发现其ATP结合位点的靶向化合物可抑制癌细胞蛋白酶体活性，为抗癌药物开发提供新策略。
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注：以上文献信息为示例性概括，实际引用请核对具体论文数据及数据库(如PubMed)。
PSMC1 (Proteasome 26S Subunit, ATPase 1) is a critical component of the 26S proteasome, a large multi-subunit protease complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMC1 functions as an ATPase that facilitates substrate recognition, deubiquitination, and unfolding of polyubiquitinated proteins prior to their translocation into the proteolytic 20S core. This ATP-dependent activity is essential for maintaining protein homeostasis, regulating cellular processes such as cell cycle progression, DNA repair, and stress response.
Recombinant PSMC1 protein is engineered using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its structural and functional properties. Its production enables detailed investigations into proteasome assembly, ATP hydrolysis mechanisms, and interactions with other proteasomal subunits or regulatory proteins. Researchers utilize recombinant PSMC1 to dissect its role in diseases linked to proteasomal dysfunction, including neurodegenerative disorders (e.g., Parkinson's and Alzheimer’s diseases) and cancers, where proteasome inhibitors are already employed therapeutically.
Structural studies using recombinant PSMC1 have revealed conserved AAA+ ATPase domains critical for forming hexameric rings that mechanically unfold substrates. Recent cryo-EM advancements further illuminated conformational changes during substrate processing. Additionally, recombinant PSMC1 serves as a tool for drug screening to identify modulators of proteasomal activity, with implications for developing targeted therapies. Its application extends to in vitro reconstitution experiments, helping decode the hierarchical assembly of the 26S proteasome and its regulatory networks in health and disease.
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