| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCG1 |
| Uniprot No | P45844 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDHKRDLGGDAEVNPFLWHRPSEEVKQTKRLKGLRKDSSSMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLR |
| 预测分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ABCG1重组蛋白的典型文献概览：
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1. **文献名称**：*ATP-binding cassette transporter G1 mediates cellular cholesterol efflux to reconstituted high-density lipoproteins*
**作者**：Wang, N., Lan, D., Chen, W., et al.
**摘要**：本研究通过哺乳动物细胞表达系统重组ABCG1蛋白，证实其通过介导胆固醇向新生高密度脂蛋白(HDL)颗粒的转运参与胆固醇逆转运过程，为动脉粥样硬化治疗提供机制依据。
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2. **文献名称**：*Purification and functional reconstitution of human ABCG1 protein in a baculovirus expression system*
**作者**：Engel, T., Lorkowski, S., Cullen, P.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达并纯化人源ABCG1重组蛋白，成功将其重构至脂质体中，验证其ATP依赖的磷脂转运活性，为体外功能研究提供可靠方法。
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3. **文献名称**：*Structural insights into ABCG1-mediated cholesterol efflux by cryo-EM*
**作者**：Lee, J.Y., Kim, K.H., Park, J., et al.
**摘要**：通过冷冻电镜解析ABCG1重组蛋白的跨膜结构域高分辨率三维结构，揭示其胆固醇结合口袋及转运机制，阐明ABCG1与ABCG家族其他成员的底物特异性差异。
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*注：以上文献标题及内容为示例性质，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"ABCG1 recombinant protein"+"cholesterol transport"/"expression"筛选近年高影响力论文。*
ABCG1 (ATP-binding cassette subfamily G member 1) is a transmembrane protein belonging to the ABC transporter superfamily, which plays a critical role in cellular lipid homeostasis. It functions as a cholesterol and phospholipid transporter, facilitating the efflux of these lipids to extracellular acceptors such as high-density lipoprotein (HDL). This process is essential for maintaining cellular cholesterol balance, particularly in macrophages, where ABCG1-mediated lipid export prevents foam cell formation, a hallmark of atherosclerosis. Dysregulation of ABCG1 has been linked to metabolic disorders, cardiovascular diseases, and neurodegenerative conditions.
Recombinant ABCG1 protein is engineered through genetic cloning and expression in heterologous systems (e.g., insect cells, mammalian cells) to study its structure, function, and therapeutic potential. Its production enables detailed biochemical characterization, including ATPase activity, lipid-binding specificity, and interaction with regulatory molecules. Researchers utilize recombinant ABCG1 to investigate its role in reverse cholesterol transport, a key anti-atherogenic mechanism, and to screen compounds that modulate its activity for drug development. Challenges in studying native ABCG1—such as low abundance and membrane localization—are overcome using recombinant versions, which provide standardized material for structural studies (e.g., cryo-EM) and functional assays. Current research focuses on understanding how ABCG1 polymorphisms affect disease susceptibility and exploring its dual role in lipid metabolism and immune response modulation. Recombinant ABCG1 also serves as a tool to decipher its crosstalk with other transporters like ABCA1. offering insights into combinatorial therapies for metabolic syndrome.
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