| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC6 |
| Uniprot No | O95255 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1503aa |
| 氨基酸序列 | MAAPAEPCAGQGVWNQTEPEPAATSLLSLCFLRTAGVWVPPMYLWVLGPIYLLFIHHHGRGYLRMSPLFKAKMVLGFALIVLCTSSVAVALWKIQQGTPEAPEFLIHPTVWLTTMSFAVFLIHTERKKGVQSSGVLFGYWLLCFVLPATNAAQQASGAGFQSDPVRHLSTYLCLSLVVAQFVLSCLADQPPFFPEDPQQSNPCPETGAAFPSKATFWWVSGLVWRGYRRPLRPKDLWSLGRENSSEELVSRLEKEWMRNRSAARRHNKAIAFKRKGGSGMKAPETEPFLRQEGSQWRPLLKAIWQVFHSTFLLGTLSLIISDVFRFTVPKLLSLFLEFIGDPKPPAWKGYLLAVLMFLSACLQTLFEQQNMYRLKVLQMRLRSAITGLVYRKVLALSSGSRKASAVGDVVNLVSVDVQRLTESVLYLNGLWLPLVWIVVCFVYLWQLLGPSALTAIAVFLSLLPLNFFISKKRNHHQEEQMRQKDSRARLTSSILRNSKTIKFHGWEGAFLDRVLGIRGQELGALRTSGLLFSVSLVSFQVSTFLVALVVFAVHTLVAENAMNAEKAFVTLTVLNILNKAQAFLPFSIHSLVQARVSFDRLVTFLCLEEVDPGVVDSSSSGSAAGKDCITIHSATFAWSQESPPCLHRINLTVPQGCLLAVVGPVGAGKSSLLSALLGELSKVEGFVSIEGAVAYVPQEAWVQNTSVVENVCFGQELDPPWLERVLEACALQPDVDSFPEGIHTSIGEQGMNLSGGQKQRLSLARAVYRKAAVYLLDDPLAALDAHVGQHVFNQVIGPGGLLQGTTRILVTHALHILPQADWIIVLANGAIAEMGSYQELLQRKGALMCLLDQARQPGDRGEGETEPGTSTKDPRGTSAGRRPELRRERSIKSVPEKDRTTSEAQTEVPLDDPDRAGWPAGKDSIQYGRVKATVHLAYLRAVGTPLCLYALFLFLCQQVASFCRGYWLSLWADDPAVGGQQTQAALRGGIFGLLGCLQAIGLFASMAAVLLGGARASRLLFQRLLWDVVRSPISFFERTPIGHLLNRFSKETDTVDVDIPDKLRSLLMYAFGLLEVSLVVAVATPLATVAILPLFLLYAGFQSLYVVSSCQLRRLESASYSSVCSHMAETFQGSTVVRAFRTQAPFVAQNNARVDESQRISFPRLVADRWLAANVELLGNGLVFAAATCAVLSKAHLSAGLVGFSVSAALQVTQTLQWVVRNWTDLENSIVSVERMQDYAWTPKEAPWRLPTCAAQPPWPQGGQIEFRDFGLRYRPELPLAVQGVSFKIHAGEKVGIVGRTGAGKSSLASGLLRLQEAAEGGIWIDGVPIAHVGLHTLRSRISIIPQDPILFPGSLRMNLDLLQEHSDEAIWAALETVQLKALVASLPGQLQYKCADRGEDLSVGQKQLLCLARALLRKTQILILDEATAAVDPGTELQMQAMLGSWFAQCTVLLIAHRLRSVMDCARVLVMDKGQVAESGSPAQLLAQKGLFYRLAQESGLV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC6重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Expression and purification of recombinant human ABCC6 in insect cells reveals ATPase activity regulated by calcium*
**作者**：A. Ilias et al.
**摘要**：研究利用杆状病毒-昆虫细胞表达系统成功表达并纯化人源ABCC6重组蛋白，证实其具有钙离子依赖的ATP酶活性，为解析ABCC6的转运功能提供了生化基础。
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2. **文献名称**：*ABCC6-mediated ATP release regulates hepatic cellular energy balance*
**作者**：R.S. Jansen et al.
**摘要**：通过重组ABCC6蛋白在哺乳动物细胞中的表达，发现其在肝细胞中通过释放ATP参与细胞能量代谢调控，并揭示了ABCC6缺失与代谢紊乱的关联。
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3. **文献名称**：*Functional characterization of ABCC6 mutations in pseudoxanthoma elasticum using recombinant protein models*
**作者**：K. Miksch et al.
**摘要**：通过构建野生型和突变型ABCC6重组蛋白，发现PXE相关突变导致蛋白转运功能受损，阐明了致病机制与ATP结合结构域的异常相关。
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如需更多文献，可进一步筛选涉及重组蛋白结构解析或治疗应用的研究。
**Background of ABCC6 Recombinant Protein**
ABCC6 (ATP-binding cassette subfamily C member 6) is a transmembrane protein primarily expressed in the liver and kidneys, playing a critical role in cellular detoxification and organic anion transport. It belongs to the ABC transporter superfamily, which utilizes ATP hydrolysis to drive substrate translocation across membranes. ABCC6 is notably associated with regulating systemic purinergic signaling by exporting ATP-derived nucleotides, such as adenosine triphosphate (ATP) and pyrophosphate (PPi), into the bloodstream. These molecules are essential for inhibiting pathological soft tissue calcification, particularly in blood vessels and elastic tissues.
Mutations in the *ABCC6* gene cause pseudoxanthoma elasticum (PXE), a rare autosomal recessive disorder characterized by ectopic mineralization in the skin, eyes, and cardiovascular system. The disease mechanism involves reduced PPi levels, leading to calcium phosphate deposition in connective tissues. Studying ABCC6's function has been challenging due to its complex post-translational modifications and tissue-specific expression.
Recombinant ABCC6 protein, produced via heterologous expression systems (e.g., mammalian cells, insect cells), enables researchers to investigate its structure, transport activity, and interaction with substrates or inhibitors. This protein is vital for elucidating molecular pathways in PXE and developing therapeutic strategies, such as enzyme replacement or small-molecule therapies to restore PPi homeostasis. Recent advances in structural biology, including cryo-EM, have provided insights into ABCC6’s conformational dynamics, aiding drug discovery efforts. However, challenges remain in achieving high-yield, functional recombinant ABCC6 due to its large size and intricate folding requirements. Ongoing research aims to refine production techniques and explore its broader physiological roles beyond mineralization disorders.
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