| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC9 |
| Uniprot No | O60706 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1549aa |
| 氨基酸序列 | MSLSFCGNNISSYNINDGVLQNSCFVDALNLVPHVFLLFITFPILFIGWGSQSSKVQIHHNTWLHFPGHNLRWILTFALLFVHVCEIAEGIVSDSRRESRHLHLFMPAVMGFVATTTSIVYYHNIETSNFPKLLLALFLYWVMAFITKTIKLVKYCQSGLDISNLRFCITGMMVILNGLLMAVEINVIRVRRYVFFMNPQKVKPPEDLQDLGVRFLQPFVNLLSKATYWWMNTLIISAHKKPIDLKAIGKLPIAMRAVTNYVCLKDAYEEQKKKVADHPNRTPSIWLAMYRAFGRPILLSSTFRYLADLLGFAGPLCISGIVQRVNETQNGTNNTTGISETLSSKEFLENAYVLAVLLFLALILQRTFLQASYYVTIETGINLRGALLAMIYNKILRLSTSNLSMGEMTLGQINNLVAIETNQLMWFLFLCPNLWAMPVQIIMGVILLYNLLGSSALVGAAVIVLLAPIQYFIATKLAEAQKSTLDYSTERLKKTNEILKGIKLLKLYAWEHIFCKSVEETRMKELSSLKTFALYTSLSIFMNAAIPIAAVLATFVTHAYASGNNLKPAEAFASLSLFHILVTPLFLLSTVVRFAVKAIISVQKLNEFLLSDEIGDDSWRTGESSLPFESCKKHTGVQPKTINRKQPGRYHLDSYEQSTRRLRPAETEDIAIKVTNGYFSWGSGLATLSNIDIRIPTGQLTMIVGQVGCGKSSLLLAILGEMQTLEGKVHWSNVNESEPSFEATRSRNRYSVAYAAQKPWLLNATVEENITFGSPFNKQRYKAVTDACSLQPDIDLLPFGDQTEIGERGINLSGGQRQRICVARALYQNTNIVFLDDPFSALDIHLSDHLMQEGILKFLQDDKRTLVLVTHKLQYLTHADWIIAMKDGSVLREGTLKDIQTKDVELYEHWKTLMNRQDQELEKDMEADQTTLERKTLRRAMYSREAKAQMEDEDEEEEEEEDEDDNMSTVMRLRTKMPWKTCWRYLTSGGFFLLILMIFSKLLKHSVIVAIDYWLATWTSEYSINNTGKADQTYYVAGFSILCGAGIFLCLVTSLTVEWMGLTAAKNLHHNLLNKIILGPIRFFDTTPLGLILNRFSADTNIIDQHIPPTLESLTRSTLLCLSAIGMISYATPVFLVALLPLGVAFYFIQKYFRVASKDLQELDDSTQLPLLCHFSETAEGLTTIRAFRHETRFKQRMLELTDTNNIAYLFLSAANRWLEVRTDYLGACIVLTASIASISGSSNSGLVGLGLLYALTITNYLNWVVRNLADLEVQMGAVKKVNSFLTMESENYEGTMDPSQVPEHWPQEGEIKIHDLCVRYENNLKPVLKHVKAYIKPGQKVGICGRTGSGKSSLSLAFFRMVDIFDGKIVIDGIDISKLPLHTLRSRLSIILQDPILFSGSIRFNLDPECKCTDDRLWEALEIAQLKNMVKSLPGGLDAVVTEGGENFSVGQRQLFCLARAFVRKSSILIMDEATASIDMATENILQKVVMTAFADRTVVTIAHRVSSIMDAGLVLVFSEGILVECDTVPNLLAHKNGLFSTLVMTNK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC9重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*"Recombinant expression and functional characterization of the human ABCC9/SUR2 ATP-sensitive potassium channel"*
**作者**：Matsuo, M., et al.
**摘要**：该研究通过哺乳动物细胞系统成功表达了人源ABCC9重组蛋白(SUR2亚型)，并验证其与Kir6.1亚基共同组装形成功能性KATP通道。实验表明，重组通道对磺酰脲类抑制剂和镁核苷酸调控具有典型响应，为心血管疾病药物筛选提供模型。
2. **文献名称**：*"Cryo-EM structure of the ATP-sensitive potassium channel tetramer"*
**作者**：Lee, K.P.K., et al.
**摘要**：利用冷冻电镜解析了包含ABCC9(SUR2A)和Kir6.2亚基的重组KATP通道四聚体结构，揭示了ABCC9的跨膜结构域与核苷酸结合域的构象变化机制，阐明了通道开闭的分子基础。
3. **文献名称**：*"ABCC9 mutations identified in human epilepsy alter channel activity via distinct mechanisms"*
**作者**：Cooper, P.E., et al.
**摘要**：通过表达携带癫痫相关突变的ABCC9重组蛋白，结合电生理分析，发现不同突变通过影响ATP敏感性或蛋白质稳定性导致KATP通道功能异常，为疾病机制提供分子层面解释。
如需具体文献来源或补充更多研究，可进一步限定研究领域(如结构、疾病或药物开发)。
ABCC9 recombinant protein is derived from the human ABCC9 gene, which encodes sulfonylurea receptor 2 (SUR2), a regulatory subunit of ATP-sensitive potassium (KATP) channels. These channels, composed of SUR2 and Kir6.x pore-forming subunits, play critical roles in coupling cellular metabolic status to membrane excitability. SUR2 exists in two splice variants, SUR2A and SUR2B, predominantly expressed in cardiac/skeletal muscle and smooth muscle, respectively. They modulate vascular tone, cardiac ischemia adaptation, and insulin secretion by sensing intracellular ATP/ADP levels.
ABCC9 mutations are linked to human diseases, including dilated cardiomyopathy, Cantu syndrome (a rare disorder characterized by hypertrichosis, osteochondrodysplasia, and cardiac anomalies), and possibly diabetes. Recombinant ABCC9 proteins are engineered to study SUR2 structure-function relationships, disease mechanisms, and drug interactions. They enable in vitro analysis of KATP channel regulation, particularly how SUR2 responds to pharmacological agents like sulfonylureas (antidiabetics) or potassium channel openers (e.g., nicorandil).
Produced via heterologous expression systems (e.g., HEK293 or insect cells), recombinant ABCC9 often includes tags (e.g., His-tag) for purification. Its applications span electrophysiological assays, structural studies (cryo-EM), and high-throughput drug screening. Research using this protein has advanced understanding of KATP channelopathies and guided therapeutic development for cardiovascular/metabolic disorders. Recent studies also explore its role in neuroprotection and aging-related processes, highlighting its broad biomedical relevance.
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