| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC2 |
| Uniprot No | Q92887 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 214-313aa |
| 氨基酸序列 | LKGYKRPLTLEDVWEVDEEMKTKTLVSKFETHMKRELQKARRALQRRQEK SSQQNSGARLPGLNKNQSQSQDALVLEDVEKKKKKSGTKKDVPKSWLMKA |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC2重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Molecular cloning and characterization of multidrug resistance-associated protein 2 (MRP2/ABCC2)*
**作者**：Buchler M, et al.
**摘要**：该研究首次克隆了人类ABCC2基因，并在哺乳动物细胞中表达重组ABCC2蛋白，证实其在细胞膜上的定位及对谷胱甘肽结合物和化疗药物的外排功能，为ABCC2的转运机制研究奠定基础。
2. **文献名称**：*Functional analysis of ABCC2 mutations using recombinant baculovirus-infected insect cells*
**作者**：Ito K, Suzuki H.
**摘要**：通过杆状病毒表达系统在昆虫细胞中重组表达ABCC2蛋白，结合突变体构建，揭示其底物结合域及关键氨基酸残基对转运活性的影响，探讨遗传变异导致的ABCC2功能障碍机制。
3. **文献名称**：*MRP2 (ABCC2) transports fluoroquinolone antibiotics via direct interaction with their protonated forms*
**作者**：Matsushima Y, et al.
**摘要**：利用重组ABCC2蛋白表达的膜囊泡模型，证明ABCC2介导氟喹诺酮类抗生素的跨膜转运，并依赖药物的质子化状态，揭示了其在药物肝肠排泄及药物相互作用中的关键作用。
4. **文献名称**：*Role of ABCC2 in drug disposition and toxicity: Lessons from in vitro and transgenic animal models*
**作者**：Nies AT, Keppler D.
**摘要**：综述性文献，总结了重组ABCC2蛋白在体外模型(如转染细胞系)及转基因动物中的应用，阐明其在药物代谢动力学、毒性和胆汁酸稳态中的作用，强调重组技术对机制解析的贡献。
这些文献涵盖了ABCC2重组蛋白的表达、功能验证及在药物转运研究中的应用，为理解其生物学和药理学意义提供了关键数据。
**Background of ABCC2 Recombinant Protein**
ABCC2 (ATP-binding cassette subfamily C member 2), also known as multidrug resistance-associated protein 2 (MRP2), is a critical transmembrane protein belonging to the ABC transporter superfamily. It plays a pivotal role in the ATP-dependent transport of endogenous and exogenous organic anions across cellular membranes. Primarily expressed in the apical membranes of hepatocytes, renal proximal tubules, and enterocytes, ABCC2 facilitates the excretion of conjugated metabolites, such as glutathione-, glucuronide-, and sulfate-conjugated compounds, into bile, urine, or the intestinal lumen. This efflux function is essential for detoxification, drug disposition, and maintaining cellular homeostasis.
Dysregulation or mutations in ABCC2 are linked to genetic disorders like Dubin-Johnson syndrome, characterized by impaired bilirubin excretion and chronic jaundice. Additionally, ABCC2 overexpression is associated with multidrug resistance in cancer cells, limiting chemotherapy efficacy by extruding anticancer agents.
Recombinant ABCC2 protein is produced via heterologous expression systems (e.g., insect, mammalian, or yeast cells) to study its structure, transport mechanisms, and interactions with substrates or inhibitors. Its purified form enables in vitro assays to elucidate substrate specificity, kinetics, and the impact of genetic variants. Researchers also use ABCC2 recombinant protein to screen drug candidates for potential transporter-mediated interactions, optimizing pharmacokinetic profiles and reducing toxicity risks.
Overall, ABCC2 recombinant protein serves as a vital tool in pharmacology, toxicology, and molecular biology, advancing our understanding of detoxification pathways and therapeutic strategies targeting ABC transporters.
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