| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC5 |
| Uniprot No | O15440 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1437aa |
| 氨基酸序列 | MKDIDIGKEYIIPSPGYRSVRERTSTSGTHRDREDSKFRRTRPLECQDALETAARAEGLSLDASMHSQLRILDEEHPKGKYHHGLSALKPIRTTSKHQHPVDNAGLFSCMTFSWLSSLARVAHKKGELSMEDVWSLSKHESSDVNCRRLERLWQEELNEVGPDAASLRRVVWIFCRTRLILSIVCLMITQLAGFSGPAFMVKHLLEYTQATESNLQYSLLLVLGLLLTEIVRSWSLALTWALNYRTGVRLRGAILTMAFKKILKLKNIKEKSLGELINICSNDGQRMFEAAAVGSLLAGGPVVAILGMIYNVIILGPTGFLGSAVFILFYPAMMFASRLTAYFRRKCVAATDERVQKMNEVLTYIKFIKMYAWVKAFSQSVQKIREEERRILEKAGYFQSITVGVAPIVVVIASVVTFSVHMTLGFDLTAAQAFTVVTVFNSMTFALKVTPFSVKSLSEASVAVDRFKSLFLMEEVHMIKNKPASPHIKIEMKNATLAWDSSHSSIQNSPKLTPKMKKDKRASRGKKEKVRQLQRTEHQAVLAEQKGHLLLDSDERPSPEEEEGKHIHLGHLRLQRTLHSIDLEIQEGKLVGICGSVGSGKTSLISAILGQMTLLEGSIAISGTFAYVAQQAWILNATLRDNILFGKEYDEERYNSVLNSCCLRPDLAILPSSDLTEIGERGANLSGGQRQRISLARALYSDRSIYILDDPLSALDAHVGNHIFNSAIRKHLKSKTVLFVTHQLQYLVDCDEVIFMKEGCITERGTHEELMNLNGDYATIFNNLLLGETPPVEINSKKETSGSQKKSQDKGPKTGSVKKEKAVKPEEGQLVQLEEKGQGSVPWSVYGVYIQAAGGPLAFLVIMALFMLNVGSTAFSTWWLSYWIKQGSGNTTVTRGNETSVSDSMKDNPHMQYYASIYALSMAVMLILKAIRGVVFVKGTLRASSRLHDELFRRILRSPMKFFDTTPTGRILNRFSKDMDEVDVRLPFQAEMFIQNVILVFFCVGMIAGVFPWFLVAVGPLVILFSVLHIVSRVLIRELKRLDNITQSPFLSHITSSIQGLATIHAYNKGQEFLHRYQELLDDNQAPFFLFTCAMRWLAVRLDLISIALITTTGLMIVLMHGQIPPAYAGLAISYAVQLTGLFQFTVRLASETEARFTSVERINHYIKTLSLEAPARIKNKAPSPDWPQEGEVTFENAEMRYRENLPLVLKKVSFTIKPKEKIGIVGRTGSGKSSLGMALFRLVELSGGCIKIDGVRISDIGLADLRSKLSIIPQEPVLFSGTVRSNLDPFNQYTEDQIWDALERTHMKECIAQLPLKLESEVMENGDNFSVGERQLLCIARALLRHCKILILDEATAAMDTETDLLIQETIREAFADCTMLTIAHRLHTVLGSDRIMVLAQGQVVEFDTPSVLLSNDSSRFYAMFAAAENKVAVKG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC5重组蛋白的3篇模拟参考文献示例：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Human ABCC5 in Insect Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达并纯化了ABCC5重组蛋白，证实其具有ATP依赖性转运活性，并证明其可外排环核苷酸cGMP，为研究ABCC5的分子机制提供了工具。
2. **文献名称**: "Structural Insights into ABCC5-Mediated Drug Resistance Using Cryo-EM"
**作者**: Thompson R, et al.
**摘要**: 通过冷冻电镜解析了ABCC5重组蛋白的3.8Å分辨率结构，揭示了其底物结合口袋的特征，阐明了与抗癌药物结合的关键氨基酸残基，为克服化疗耐药性提供了结构基础。
3. **文献名称**: "Development of a High-Throughput Screening Assay for ABCC5 Inhibitors Using Recombinant Protein"
**作者**: Chen X, et al.
**摘要**: 建立基于ABCC5重组蛋白的荧光底物外排筛选体系，鉴定了新型小分子抑制剂NP-12.可显著增强化疗药物在耐药细胞系中的积累，验证了靶向ABCC5的逆转耐药策略。
(注：以上文献为模拟数据，实际研究中建议通过PubMed/Google Scholar检索关键词"ABCC5 recombinant protein"获取真实文献)
ABCC5 (ATP-binding cassette subfamily C member 5), also known as multidrug resistance-associated protein 5 (MRP5), is a transmembrane transporter protein belonging to the ATP-binding cassette (ABC) superfamily. It plays a critical role in cellular efflux of endogenous molecules and xenobiotics, including cyclic nucleotides (cAMP, cGMP), antiviral drugs, anticancer agents, and organic anions. ABCC5 is widely expressed in tissues such as the liver, kidneys, blood-brain barrier, and placenta, highlighting its involvement in drug disposition, detoxification, and cellular signaling pathways.
Recombinant ABCC5 protein refers to the engineered form of this transporter produced in heterologous expression systems (e.g., mammalian cells, insect cells) for functional and structural studies. Its production enables detailed investigations into substrate specificity, transport mechanisms, and interactions with pharmacological inhibitors. Research on recombinant ABCC5 has revealed its role in multidrug resistance (MDR) in cancers, where overexpression reduces intracellular drug accumulation, compromising chemotherapy efficacy. Additionally, ABCC5-mediated cGMP export influences cardiovascular and neurological functions, linking it to diseases like hypertension and Alzheimer’s.
Structural studies using recombinant ABCC5 have provided insights into its ATP-dependent transport cycle and substrate-binding domains, aiding the design of modulators to overcome drug resistance or enhance therapeutic delivery. Its application extends to in vitro models for assessing drug-drug interactions, toxicity, and pharmacokinetics. Recent advances in cryo-EM and mutagenesis approaches using recombinant protein have further elucidated its conformational dynamics and regulatory mechanisms. As ABCC5 intersects with critical physiological and pathological processes, its recombinant form remains a vital tool for advancing drug development and understanding disease biology.
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