| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPATCH3 |
| Uniprot No | Q96I76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-525aa |
| 氨基酸序列 | MAVPGEAEEE ATVYLVVSGI PSVLRSAHLR SYFSQFREER GGGFLCFHYR HRPERAPPQA APNSALIPTD PAAEGQLLSQ TSATDVRPLS TRDSTPIQTR TCCCVISVRG LAQAQRLIRM YSGRRWLDSH GTWLPGRCLI RRLRLPTEAS GLGSFPFKTR KELQSWKAEN EAFTLADLKQ LPELNPPVLM PRGNVGTPLR VFLELIRACR LPPRIITQLQ LQFPKTGSSR RYGNVPFEYE DSETVEQEEL VYTAEGEEIP QGTYLADIPA SPCGEPEEEV GKEEEEESHS DEDDDRGEEW ERHEALHEDV TGQERTTEQL FEEEIELKWE KGGSGLVFYT DAQFWQEEEG DFDEQTADDW DVDMSVYYDR DGGDKDARDS VQMRLEQRLR DGQEDGSVIE RQVGTFERHT KGIGRKVMER QGWAEGQGLG CRCSGVPEAL DSDGQHPRCK RGLGYHGEKL QPFGQLKRPR RNGLGLISTI YDEPLPQDQT ESLLRRQPPT SMKFRTDMAF VRGSSCASDS PSLPD |
| 分子量 | 85.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPATCH3蛋白的模拟参考文献及摘要概括，基于领域内相关研究的常见方向推测：
1. **文献名称**：**"Cloning, expression, and functional characterization of recombinant human GPATCH3 protein in RNA metabolism"**
**作者**：Lee S, et al.
**摘要**：研究报道了GPATCH3的基因克隆、原核/真核表达系统优化及蛋白纯化，发现其通过G-patch结构域结合RNA解旋酶DDX5.调控mRNA剪接过程，并证明重组GPATCH3在体外增强DDX5的ATP酶活性。
2. **文献名称**：**"GPATCH3 promotes hepatocellular carcinoma proliferation by interacting with CTNNB1"**
**作者**：Zhang Y, et al.
**摘要**：通过重组GPATCH3蛋白的体外实验，结合免疫共沉淀和蛋白质组学分析，揭示其与β-catenin(CTNNB1)的相互作用，激活Wnt信号通路，促进肝癌细胞增殖及迁移，提示其作为潜在治疗靶点。
3. **文献名称**：**"Structural insights into the RNA-binding domain of human GPATCH3 by X-ray crystallography"**
**作者**：Müller P, et al.
**摘要**：首次解析了GPATCH3的G-patch结构域晶体结构(2.1 Å)，揭示其保守的疏水核心和带正电荷表面，并通过点突变验证其结合RNA/DNA的能力，为设计功能抑制剂奠定基础。
4. **文献名称**：**"GPATCH3 deficiency impairs spermatogenesis via aberrant mitochondrial dynamics"**
**作者**：Chen X, et al.
**摘要**：在小鼠模型中发现GPATCH3敲除导致雄性不育，使用重组人GPATCH3回补可部分恢复线粒体形态，证明其通过调控RUVBL1复合物维持线粒体融合-分裂平衡，影响精子形成。
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**注**：以上文献为模拟示例，实际发表情况需通过学术数据库核实。GPATCH3研究尚处于早期，建议通过PubMed或Web of Science以**“GPATCH3”**及**“recombinant”**组合检索最新进展。若需真实文献，可提供检索方法指导。
**Background of Recombinant Human GPATCH3 Protein**
Recombinant human GPATCH3 protein is a genetically engineered version of the naturally occurring GPATCH3 (G-patch domain-containing protein 3) encoded by the *GPATCH3* gene in humans. This protein belongs to the G-patch domain family, characterized by a conserved glycine-rich motif implicated in RNA binding and nucleic acid metabolism. GPATCH3 is ubiquitously expressed in tissues, with higher levels observed in the testis, skeletal muscle, and brain, suggesting roles in diverse biological processes. While its precise molecular function remains under investigation, GPATCH3 is thought to participate in RNA processing, including splicing or modification, potentially interacting with RNA-associated complexes like the LSm14A-containing granules involved in mRNA regulation.
The recombinant form is typically produced in *E. coli* or mammalian expression systems, ensuring high purity and activity for experimental use. Its production enables researchers to study GPATCH3's interactions, structural properties, and regulatory mechanisms in vitro or in cellular models. Current research links GPATCH3 to cancer progression, neurological disorders, and germ cell development, though its exact pathological roles are unclear. Recombinant GPATCH3 serves as a critical tool for elucidating its biological significance and exploring therapeutic targets in diseases associated with RNA dysregulation. Further studies are needed to map its interactome and define its contributions to cellular homeostasis.
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