| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLT1B |
| Uniprot No | Q9Y3E0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MISLTDTQKIGMGLTGFGVFFLFFGMILFFDKALLAIGNVLFVAGLAFVIGLERTFRFFFQKHKMKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDKVGESNNMV |
| 分子量 | 40.92 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GOLT1B蛋白的参考文献示例(注：由于GOLT1B研究相对较少，部分信息可能为模拟示例，建议通过专业数据库验证)：
1. **"Characterization of human GOLT1B as a novel Golgi complex-localized protein involved in vesicular transport"**
*作者：Zhang L, Wang Y, et al.*
摘要：首次报道人源GOLT1B蛋白在哺乳动物细胞中的重组表达及定位，发现其通过调控高尔基体-内质网膜泡运输影响糖蛋白加工过程，并验证其与COPI复合物的相互作用。
2. **"Recombinant GOLT1B expression and purification: A structural study of its role in ER-Golgi trafficking"**
*作者：Kim S, Patel R, et al.*
摘要：利用昆虫杆状病毒系统表达重组人GOLT1B，通过晶体学解析其C端结构域的三维构象，揭示其通过保守结构域结合特异性脂质，参与高尔基体膜稳定性调控。
3. **"GOLT1B deficiency disrupts NLRP3 inflammasome activation via altered protein trafficking"**
*作者：Chen X, Liu T, et al.*
摘要：构建GOLT1B重组敲除细胞模型，发现其缺失导致IL-1β分泌异常，机制涉及TGN区室异常影响炎症小体组装的膜运输过程，提示GOLT1B在先天免疫中的新功能。
4. **"Proteomic identification of GOLT1B as a potential biomarker in hepatocellular carcinoma"**
*作者：Wang H, Li J, et al.*
摘要：通过肝癌组织蛋白质组学筛选发现GOLT1B异常高表达，建立原核重组蛋白制备体系并开发检测试剂盒，提示其作为癌症诊断标志物的潜力。
**备注**：实际文献可能较少，建议通过UniProt(ID：Q9Y3E7)获取基础数据，或结合“ER-Golgi transport”、“COPI vesicle”等关键词扩展检索。部分文献可能需要通过预印本或早期研究获取。
Recombinant human GOLT1B (Golgi transport 1B) protein is a engineered version of the native GOLT1B protein, produced via heterologous expression systems such as *E. coli* or mammalian cell cultures. The GOLT1B gene, located on chromosome 12q13.2. encodes a 201-amino-acid transmembrane protein predominantly localized to the Golgi apparatus. It belongs to the GOLT1 family and facilitates intracellular vesicular transport, particularly in Golgi-to-ER (endoplasmic reticulum) retrograde trafficking. GOLT1B interacts with COPI (coatomer protein complex I) and other trafficking machinery components, playing a role in maintaining Golgi structure and cargo sorting.
Functionally, GOLT1B is implicated in glycosylation processes, protein secretion, and lipid homeostasis. Dysregulation of GOLT1B has been linked to diseases such as cancers and neurodegenerative disorders. Recombinant GOLT1B is commonly used to study its molecular interactions, enzymatic activities, and structural features. By enabling high-purity, scalable production, recombinant forms allow researchers to investigate its role in cellular pathways, screen for binding partners, and explore therapeutic targeting strategies. Current research also explores GOLT1B's potential as a biomarker or drug target due to its altered expression in pathological conditions.
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