| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLPH4 |
| Uniprot No | O00461 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 473-568aa |
| 氨基酸序列 | HQEQLRQQAHYDAMDNDIVQGAEDQGIQGEEGAYERDNQHQDEAEGDPGNRHEPREQGPREADPESEADRAAVEDINPADDPNNQGEDEFEEAEQV |
| 分子量 | 36.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GOLPH4蛋白的示例参考文献(注：文献为假设性示例，仅供格式参考)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human GOLPH4 in Golgi Trafficking"*
**作者**: Zhang et al.
**摘要**: 研究报道了重组人GOLPH4蛋白在HEK293细胞中的真核表达与纯化，发现其通过与高尔基体膜蛋白相互作用，调控蛋白质的分选和运输。
2. **文献名称**: *"GOLPH4 Promotes Tumor Progression by Modulating EGFR Recycling in Breast Cancer"*
**作者**: Wang et al.
**摘要**: 通过构建重组GOLPH4蛋白，揭示了其在乳腺癌细胞中过表达的机制，表明GOLPH4通过调控EGFR内吞和再循环增强下游信号通路，促进肿瘤侵袭。
3. **文献名称**: *"Structural Insights into GOLPH4-Mediated Membrane Reshaping via Cryo-EM Analysis"*
**作者**: Chen et al.
**摘要**: 利用冷冻电镜解析了重组GOLPH4蛋白的三维结构，提出其通过结合磷脂酰肌醇磷酸(PIP)调控高尔基体膜形态的分子机制。
4. **文献名称**: *"GOLPH4 as a Novel Regulator of Autophagy: Implications in Neurodegeneration"*
**作者**: Kim et al.
**摘要**: 研究发现重组GOLPH4蛋白通过促进自噬体形成缓解神经元蛋白聚集，提示其在神经退行性疾病中的潜在治疗作用。
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如需真实文献，建议通过PubMed或Google Scholar以关键词“GOLPH4 recombinant protein”、“GOLIM4 function”检索。部分已知研究中，GOLPH4可能与病毒出胞(如VSV-G运输)或癌症生物标志物相关。
Recombinant human GOLPH4 (Golgi Phosphoprotein 4), also known as Golgin-97 or GOLM1. is a peripheral membrane protein predominantly localized to the Golgi apparatus. It plays a critical role in maintaining Golgi structure, vesicular trafficking, and cargo sorting. GOLPH4 interacts with components of the retrograde transport machinery, including the Arf1 GTPase and the COPI complex, facilitating the recycling of proteins between the Golgi and endoplasmic reticulum. Structurally, it contains a conserved GRIP domain at its C-terminus, which mediates Golgi targeting, and coiled-coil regions for oligomerization.
Dysregulation of GOLPH4 has been implicated in cancer progression, neurodegenerative diseases, and viral infections. For example, elevated GOLPH4 expression correlates with tumor aggressiveness in hepatocellular carcinoma and prostate cancer. Recombinant GOLPH4 protein is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications. Its applications span structural studies, interaction mapping with transport machinery, and screening for inhibitors targeting Golgi-associated pathways. Research on recombinant GOLPH4 enhances understanding of Golgi dynamics and may lead to therapeutic strategies for diseases linked to Golgi dysfunction.
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