| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGA6A |
| Uniprot No | Q9NYA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693aa |
| 氨基酸序列 | MWPQPYLPPHPMMLEESRQNKLAAAKKKLKEYQQRKSPGIPAGAKTKKKKTDSSPETTTSGGCHSPGDSQYQELAVALESSSVTISQLNENIESLKQQKKQVEHQLEEAKKTNNEIHKAQMERLETINILTLEKADLKTTLYHTKRAARHFEEESKDLAGRLQYSLQRIQELERALCAVSTQQQEEDRSSSCREAVLQRWLQQTIKERALLNAHVTQVTESLKQVQLERDEYAKHIKGERARWQERMWKMSVEARTLKEEKKRDIHRIQELERSLSELKNQMAEPPSLAPPAVTSVVEQLQDEAKHLRQEVEGLEGKLQSQVENNQALSLLSKEQKQRLQEQEEMLREQEAQRVREQERLCEQNERLREQQKTLQEQGERLRKQEQRLRKQEERLRKEEERLQKQEKRLWDQEERLWKKEERLQKQEERLALSQNHKLDKQLAEPQCSFEDLNNEKKSALQLEQQVKELQEKLDEEHLEAASHQNQQLETQLSLVALPGEGDGGQHLDSEEEEAPRPTPNIPEDLESREATSSFMDLPKEKADGTEQVERRELGFVQPSGVTDGMRESFTVYESQGAVPNTRHQEMEDVIRLAQKEEEMKVKLLELQELVLPLVGNHEGHGKFLIAAQNPADEPTPGAPAPQELGAAGEQDVFYEVSLDNNVEPAPGAAREGSPHDNPTVQQIVQLSPVMQDT |
| 分子量 | 76.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GOLGA6A蛋白的参考文献及其简要内容(注：以下信息基于假设性文献，实际文献需通过学术数据库验证)：
1. **文献名称**: *"Recombinant Expression and Functional Analysis of Human GOLGA6A in Golgi Apparatus Organization"*
**作者**: Smith J, et al.
**摘要**: 研究报道了在HEK293细胞中重组表达人GOLGA6A蛋白，通过荧光标记技术发现其参与调控高尔基体的形态和囊泡运输，并揭示了其与COPI复合物的潜在相互作用。
2. **文献名称**: *"Purification and Biochemical Characterization of Recombinant GOLGA6A for Antibody Development"*
**作者**: Zhang L, et al.
**摘要**: 利用大肠杆菌表达系统获得高纯度重组GOLGA6A蛋白，优化了纯化条件，并基于该蛋白制备了特异性抗体，为后续疾病标志物研究提供工具。
3. **文献名称**: *"GOLGA6A Gene Duplication in Primates: Implications for Recombinant Protein Evolution Studies"*
**作者**: Brown K, et al.
**摘要**: 分析了GOLGA6A基因在灵长类中的复制事件，通过重组蛋白比对揭示其结构域保守性差异，推测其在物种特异性细胞功能中可能的作用。
4. **文献名称**: *"Role of Recombinant GOLGA6A in Cancer Cell Adhesion and Metastasis"*
**作者**: Lee S, et al.
**摘要**: 通过体外实验验证重组GOLGA6A蛋白能够增强肿瘤细胞的迁移能力，可能与整合素信号通路相关，提示其在癌症转移中的潜在机制。
建议通过PubMed或Google Scholar以关键词“GOLGA6A recombinant”“GOLGA6A expression”检索真实文献，并结合EndNote等工具管理参考文献。
GOLGA6A, a member of the Golgin A protein family, is a human gene encoding a protein predominantly localized to the Golgi apparatus. This organelle-specific protein is hypothesized to play a role in maintaining Golgi structure, vesicular trafficking, and protein processing. While its precise biological function remains incompletely characterized, GOLGA6A shares conserved structural features with other Golgins, including coiled-coil domains that mediate interactions with membranes and other cellular components. Studies suggest potential involvement in spermatogenesis, as it is selectively expressed in germ cells, though further validation is required.
Recombinant human GOLGA6A protein is produced through heterologous expression systems (e.g., mammalian cells or E. coli) to enable functional studies. Purified recombinant forms facilitate investigations into its biochemical properties, interactome mapping, and antibody development. Clinically, aberrant GOLGA6A expression has been loosely associated with cancers, raising interest in its utility as a biomarker or therapeutic target. However, research remains nascent, hampered by challenges such as sequence homology with other GOLGA6 family paralogs (e.g., GOLGA6B, 6C) and undefined signaling pathways.
Current applications focus on elucidating its role in Golgi dynamics and disease mechanisms, emphasizing the need for advanced structural and in vivo studies to clarify its functional significance.
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