| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GORASP1 |
| Uniprot No | Q9BQQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440aa |
| 氨基酸序列 | MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPPSYHKKPPGTPPPSALPLGAPPPDALPPGPTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPSHSAPDPDGLPHFMETPLQPPPPVQRVMDPGFLDVSGISLLDNSNASVWPSLPSSTELTTTAVSTSGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE |
| 分子量 | 72.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人GORASP1蛋白相关的模拟参考文献示例(内容基于领域知识综合虚构，供参考)：
1. **文献名称**：**"GRASP55/GORASP1 phosphorylation regulates Golgi structure and protein trafficking in mammalian cells"**
**作者**：Wang Y. et al.
**摘要**：研究通过重组表达人源GORASP1蛋白，分析其磷酸化对高尔基体结构及分泌通路的影响，证实磷酸化修饰通过调控其与GRASP55的相互作用，参与细胞分裂后高尔基体重组。
2. **文献名称**：**"Recombinant human GORASP1 facilitates ER-to-Golgi transport by tethering vesicles in vitro"**
**作者**：Zhang L. et al.
**摘要**：体外实验表明重组GORASP1蛋白通过C端结构域介导囊栓聚，增强内质网来源的运输小泡与高尔基体膜融合效率，揭示了其在早期分泌通路中的分子机制。
3. **文献名称**：**"GORASP1 knockdown impairs collagen secretion and promotes metastasis in breast cancer models"**
**作者**：Chen X. et al.
**摘要**：利用重组GORASP1蛋白补充实验，证明其缺失导致乳腺癌细胞胶原分泌障碍，促进上皮-间质转化(EMT)，提示其在肿瘤微环境调控中的潜在作用。
4. **文献名称**：**"Structural insights into the dimerization of GRASP family proteins: Cryo-EM analysis of human GORASP1"**
**作者**：Tanaka R. et al.
**摘要**：通过冷冻电镜解析重组GORASP1蛋白二聚体结构，揭示其PDZ结构域介导的自组装机制，为靶向高尔基体形态异常疾病的药物设计提供依据。
(注：上述文献为示例，实际研究中请通过PubMed或专业数据库核实真实文献。)
Golgi reassembly-stacking protein 1 (GORASP1), also known as GOLPH1 or GRASP65. is a conserved scaffolding protein critical for maintaining the structure and function of the Golgi apparatus. It plays a pivotal role in Golgi stack formation, vesicle tethering, and post-translational modification of secretory proteins. GORASP1 contains a GRASP domain that enables homodimerization and membrane association, facilitating Golgi cisternae stacking. During cell division, it mediates Golgi membrane reorganization and partitioning into daughter cells. Dysregulation of GORASP1 has been linked to impaired protein secretion, altered autophagy, and diseases including cancer and neurodegenerative disorders.
Recombinant human GORASP1 protein is typically produced in bacterial or mammalian expression systems, enabling biochemical studies of its interactions with Golgi components, regulatory kinases (e.g., CDK1. MAPK), and cargo receptors. Its purified form supports in vitro investigations of Golgi reassembly mechanisms, membrane fusion dynamics, and stress-induced Golgi fragmentation. Researchers utilize recombinant GORASP1 to develop assays for drug candidates targeting secretory pathway disorders and to study viral strategies that exploit Golgi trafficking (e.g., coronavirus replication). Structural analysis of recombinant GORASP1 has revealed phosphorylation-dependent conformational changes regulating its membrane attachment during cell cycle progression.
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