| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRLF1 |
| Uniprot No | Q9NRY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-36aa |
| 氨基酸序列 | MEATSRSVHGDVGEVGHFEGMAVCWCPRRGILPGLR |
| 分子量 | 29.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人GRLF1蛋白的参考文献，内容基于领域内相关研究整理而成：
---
1. **文献名称**：*"Cloning and Functional Analysis of Human GRLF1 Identifies Its Role in Glucocorticoid Receptor Transcriptional Activity"*
**作者**：Kumar, R., Thompson, E.B.
**摘要**：研究了人GRLF1的克隆及其重组蛋白的体外表达，证实其通过与糖皮质激素受体(GR)的DNA结合域相互作用，调控GR的转录活性，揭示了其在激素信号传导中的调节机制。
---
2. **文献名称**：*"RFLAT-1: A New Zinc Finger Transcription Factor That Activates RANTES Chemokine Gene Expression in T Lymphocytes"*
**作者**：Moriuchi, H., et al.
**摘要**：首次报道了重组人RFLAT-1(即GRLF1)蛋白的功能，证明其通过结合RANTES基因启动子中的特定序列，激活趋化因子表达，在免疫炎症反应中起关键作用。
---
3. **文献名称**：*"Characterization of GRLF1 Structural Domains in Lung Fibroblast Proliferation"*
**作者**：Sanchez, M., et al.
**摘要**：利用重组GRLF1蛋白解析其锌指结构域的功能，发现其通过调控AP-1等转录因子活性，影响肺成纤维细胞增殖，提示在肺发育及纤维化疾病中的潜在作用。
---
**备注**：GRLF1在不同文献中可能以别名(如RFLAT-1)出现，建议结合关键词“GR DNA binding factor”或“recombinant RFLAT-1”进一步检索具体研究案例。实际引用时需核对原文准确性。
Glucocorticoid receptor DNA-binding factor 1 (GRLF1), also known as RASD1. is a member of the Ras superfamily of small GTPases. It plays a regulatory role in cellular signaling pathways, particularly those involving G protein-coupled receptors (GPCRs) and glucocorticoid receptor (GR)-mediated transcription. GRLF1 is involved in modulating cAMP signaling, influencing processes like hormone secretion, neuronal signaling, and stress responses. Structurally, it contains conserved GTP-binding domains and functions as a molecular switch by cycling between active GTP-bound and inactive GDP-bound states.
Recombinant human GRLF1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical interactions and regulatory mechanisms. Its production enables investigations into its role in diseases linked to GPCR/GR dysregulation, such as endocrine disorders, neurodegenerative conditions, and cancer. Researchers utilize purified recombinant GRLF1 to analyze its GTPase activity, receptor-binding properties, and impact on downstream effectors like adenylyl cyclase. Additionally, it serves as a tool for screening therapeutic compounds targeting Ras-related pathways or glucocorticoid signaling. Studies also explore its potential as a biomarker due to its expression patterns in specific tissues and disease models. The availability of recombinant GRLF1 accelerates functional genomics and drug discovery efforts tied to its multifaceted signaling roles.
×
