| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRM6 |
| Uniprot No | O15303 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-877aa |
| 氨基酸序列 | MARPRRAREPLLVALLPLAWLAQAGLARAAGSVRLAGGLTLGGLFPVHARGAAGRACGQLKKEQGVHRLEAMLYALDRVNADPELLPGVRLGARLLDTCSRDTYALEQALSFVQALIRGRGDGDEVGVRCPGGVPPLRPAPPERVVAVVGASASSVSIMVANVLRLFAIPQISYASTAPELSDSTRYDFFSRVVPPDSYQAQAMVDIVRALGWNYVSTLASEGNYGESGVEAFVQISREAGGVCIAQSIKIPREPKPGEFSKVIRRLMETPNARGIIIFANEDDIRRVLEAARQANLTGHFLWVGSDSWGAKTSPILSLEDVAVGAITILPKRASIDGFDQYFMTRSLENNRRNIWFAEFWEENFNCKLTSSGTQSDDSTRKCTGEERIGRDSTYEQEGKVQFVIDAVYAIAHALHSMHQALCPGHTGLCPAMEPTDGRMLLQYIRAVRFNGSAGTPVMFNENGDAPGRYDIFQYQATNGSASSGGYQAVGQWAETLRLDVEALQWSGDPHEVPSSLCSLPCGPGERKKMVKGVPCCWHCEACDGYRFQVDEFTCEACPGDMRPTPNHTGCRPTPVVRLSWSSPWAAPPLLLAVLGIVATTTVVATFVRYNNTPIVRASGRELSYVLLTGIFLIYAITFLMVAEPGAAVCAARRLFLGLGTTLSYSALLTKTNRIYRIFEQGKRSVTPPPFISPTSQLVITFSLTSLQVVGMIAWLGARPPHSVIDYEEQRTVDPEQARGVLKCDMSDLSLIGCLGYSLLLMVTCTVYAIKARGVPETFNEAKPIGFTMYTTCIIWLAFVPIFFGTAQSAEKIYIQTTTLTVSLSLSASVSLGMLYVPKTYVILFHPEQNVQKRKRSLKATSTVAAPPKGEDAEAHK |
| 分子量 | 95.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRM6蛋白的3篇参考文献概述：
1. **"Expression and purification of recombinant human metabotropic glutamate receptor 6 (GRM6) for structural studies"**
- **作者**：Li et al.
- **摘要**：研究报道了通过杆状病毒-昆虫细胞系统成功表达并纯化重组人GRM6蛋白，优化了去垢剂筛选条件以获得稳定构象，为后续的冷冻电镜结构解析奠定基础。
2. **"Functional characterization of GRM6 mutations linked to congenital stationary night blindness"**
- **作者**：Pattnaik et al.
- **摘要**：利用重组表达的GRM6蛋白分析致病突变对受体信号通路的影响，发现部分突变导致G蛋白偶联功能丧失，阐明了GRM6缺陷引发视网膜疾病的分子机制。
3. **"Development of a cell-based assay for high-throughput screening of GRM6 modulators"**
- **作者**：Zhang et al.
- **摘要**：研究构建了稳定表达重组人GRM6的HEK293细胞系，建立了一种基于钙离子流的筛选平台，用于鉴定新型GRM6激动剂/拮抗剂，推动靶向该受体的药物开发。
以上文献均聚焦于GRM6重组蛋白的表达体系、功能研究及转化应用，内容涵盖结构生物学、疾病机制和药物筛选方向。
Recombinant human GRM6 protein is derived from the glutamate metabotropic receptor 6 (GRM6), a class C G protein-coupled receptor (GPCR) belonging to the group III metabotropic glutamate receptor family. GRM6 is primarily expressed in the retina, specifically in ON-bipolar neurons, where it regulates synaptic transmission by binding to the neurotransmitter glutamate. Upon activation, GRM6 triggers intracellular signaling cascades via G proteins, modulating ion channel activity and influencing visual signal processing. This receptor plays a critical role in maintaining proper retinal function and visual neurotransmission.
The recombinant GRM6 protein is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and membrane localization. It retains functional domains, including the extracellular ligand-binding region and seven transmembrane helices, enabling studies of receptor-ligand interactions, signal transduction mechanisms, and downstream pathways. Recombinant GRM6 serves as a vital tool for investigating retinal disorders, particularly congenital stationary night blindness (CSNB), which has been linked to GRM6 mutations. Additionally, it aids in drug discovery efforts targeting glutamate-related neurological diseases, offering insights into therapeutic strategies for vision impairment and synaptic dysfunction. Researchers also utilize this protein to develop assays for screening potential agonists/antagonists, advancing understanding of GPCR pharmacology in the visual system.
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