| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRM7 |
| Uniprot No | Q14831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-915aa |
| 氨基酸序列 | MVQLRKLLRVLTLMKFPCCVLEVLLCALAAAARGQEMYAPHSIRIEGDVTLGGLFPVHAKGPSGVPCGDIKRENGIHRLEAMLYALDQINSDPNLLPNVTLGARILDTCSRDTYALEQSLTFVQALIQKDTSDVRCTNGEPPVFVKPEKVVGVIGASGSSVSIMVANILRLFQIPQISYASTAPELSDDRRYDFFSRVVPPDSFQAQAMVDIVKALGWNYVSTLASEGSYGEKGVESFTQISKEAGGLCIAQSVRIPQERKDRTIDFDRIIKQLLDTPNSRAVVIFANDEDIKQILAAAKRADQVGHFLWVGSDSWGSKINPLHQHEDIAEGAITIQPKRATVEGFDAYFTSRTLENNRRNVWFAEYWEENFNCKLTISGSKKEDTDRKCTGQERIGKDSNYEQEGKVQFVIDAVYAMAHALHHMNKDLCADYRGVCPEMEQAGGKKLLKYIRNVNFNGSAGTPVMFNKNGDAPGRYDIFQYQTTNTSNPGYRLIGQWTDELQLNIEDMQWGKGVREIPASVCTLPCKPGQRKKTQKGTPCCWTCEPCDGYQYQFDEMTCQHCPYDQRPNENRTGCQDIPIIKLEWHSPWAVIPVFLAMLGIIATIFVMATFIRYNDTPIVRASGRELSYVLLTGIFLCYIITFLMIAKPDVAVCSFRRVFLGLGMCISYAALLTKTNRIYRIFEQGKKSVTAPRLISPTSQLAITSSLISVQLLGVFIWFGVDPPNIIIDYDEHKTMNPEQARGVLKCDITDLQIICSLGYSILLMVTCTVYAIKTRGVPENFNEAKPIGFTMYTTCIVWLAFIPIFFGTAQSAEKLYIQTTTLTISMNLSASVALGMLYMPKVYIIIFHPELNVQKRKRSFKAVVTAATMSSRLSHKPSDRPNGEAKTELCENVDPNSPAAKKKYVSYNNLVI |
| 分子量 | 128.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GRM7蛋白的模拟参考文献示例(内容基于领域内常见研究方向综合描述，非真实文献)：
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1. **文献名称**: *"Cloning and Functional Characterization of Human Metabotropic Glutamate Receptor 7 (GRM7)"*
**作者**: Yao et al.
**摘要**: 研究报道了重组人GRM7蛋白的克隆、哺乳动物细胞表达及其药理学特性，揭示了其在调节突触谷氨酸信号传递中的负反馈作用。
2. **文献名称**: *"GRM7 Deficiency Alters Anxiety-Related Behaviors in Mice via Dysregulated Synaptic Plasticity"*
**作者**: Smith et al.
**摘要**: 通过构建GRM7基因敲除小鼠模型，发现GRM7蛋白缺失导致焦虑样行为增加，并证明其通过mGluR7受体调控海马区突触可塑性异常相关机制。
3. **文献名称**: *"Structural Insights into Human GRM7 Activation by Cryo-EM"*
**作者**: Wang et al.
**摘要**: 利用冷冻电镜技术解析了重组人GRM7蛋白与负变构调节剂结合的高分辨率结构，阐明了其跨膜结构域变构调控机制，为靶向药物设计提供依据。
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**注**: 以上为模拟参考文献，实际文献需通过PubMed或Google Scholar检索关键词“human GRM7 recombinant”“mGluR7”等获取。
Recombinant human GRM7 protein is a engineered form of the metabotropic glutamate receptor 7 (mGlu7), a class C G protein-coupled receptor (GPCR) predominantly expressed in the central nervous system. As a member of the group III metabotropic glutamate receptors, GRM7 is activated by glutamate, the primary excitatory neurotransmitter, and modulates synaptic transmission via Gαi/o protein-coupled signaling pathways. It plays a critical role in regulating neurotransmitter release, synaptic plasticity, and neuronal excitability, contributing to processes such as learning, memory, and emotional regulation. Dysregulation of GRM7 has been implicated in neurological and psychiatric disorders, including anxiety, depression, schizophrenia, and autism spectrum disorders.
The recombinant protein is typically produced in heterologous expression systems (e.g., HEK293 or insect cells) to enable structural and functional studies. It retains key domains, including the large extracellular ligand-binding region, seven-transmembrane helices, and intracellular signaling domains, allowing researchers to investigate receptor-ligand interactions, downstream signaling mechanisms, and potential therapeutic targets. Recombinant GRM7 serves as a vital tool for drug discovery, particularly in developing modulators to treat disorders linked to glutamate signaling abnormalities. Its application spans crystallography, electrophysiology, and high-throughput screening assays aimed at understanding receptor pharmacology and neuropathophysiology.
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