| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSG1 |
| Uniprot No | Q2KHT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MELSKAFSGQRTLLSAILSMLSLSFSTTSLLSNYWFVGTQKVPKPLCEKGLAAKCFDMPVSLDGDTNTSTQEVVQYNWETGDDRFSFRSFRSGMWLSCEETVEEPGERCRSFIELTPPAKRGEKGLLEFATLQGPCHPTLRFGGKRLMEKASLPSPPLGLCGKNPMVIPGNADHLHRTSIHQLPPATNRLATHWEPCLWAQTERLCCCFLCPVRSPGDGGPHDVFTSLPSDCQLGSRRLETTCLELWLGLLHGLALLHLLHGVGCHHLQHVHQDGAGVQVQA |
| 分子量 | 57.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我根据学术文献格式模拟的关于重组人GSG1蛋白的研究案例(注：由于GSG1蛋白研究较新且文献有限，以下内容为示例性虚构，建议通过PubMed或Web of Science等平台核实真实文献)：
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1. **文献名称**：Structural Characterization of Recombinant Human GSG1 and Its Role in Spermatogenesis
**作者**：Zhang L, et al.
**摘要**：本研究首次在大肠杆菌系统中表达并纯化了重组人GSG1蛋白，通过X射线晶体学解析其三维结构(分辨率为2.1 Å)，发现其具有典型的RNA结合域。功能实验表明，GSG1在小鼠精子发生过程中调控mRNA稳定性。
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2. **文献名称**：GSG1 Interaction with PIWI Proteins in Germline Cells
**作者**：Tanaka K, et al.
**摘要**：利用免疫共沉淀和酵母双杂交技术，发现重组人GSG1蛋白与PIWI家族蛋白(如HIWI)在人类睾丸细胞中存在直接相互作用，可能通过piRNA通路参与生殖细胞发育异常疾病的调控。
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3. **文献名称**：Development of a GSG1 Knockout Mouse Model Using CRISPR/Cas9
**作者**：Wang Y, et al.
**摘要**：构建了GSG1基因敲除小鼠模型，发现GSG1缺失导致雄性不育。通过回补实验证明，注射重组人GSG1蛋白可部分恢复精子形态异常，提示其临床治疗潜力。
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4. **文献名称**：High-Throughput Screening of GSG1 Inhibitors for Cancer Therapy
**作者**：Miller R, et al.
**摘要**：基于重组GSG1蛋白建立高通量药物筛选平台，筛选出小分子抑制剂NP-203.可特异性阻断GSG1与RNA解旋酶DDX4的结合，在体外抑制睾丸癌细胞增殖(IC50=3.2 μM)。
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**注意**：以上文献为模拟案例，实际研究请参考真实数据库。若需查找真实文献，建议使用关键词“GSG1 protein recombinant human”或“GSG1 gene function”在以下平台检索：
- PubMed (https://pubmed.ncbi.nlm.nih.gov)
- Google Scholar (https://scholar.google.com)
Recombinant human GSG1 (Germ Cell-Specific Gene 1) protein is a biologically engineered molecule derived from the GSG1 gene, which is predominantly expressed in germ cells and plays critical roles in reproductive biology. GSG1 encodes a serine/threonine-rich protein implicated in gametogenesis, particularly during spermatogenesis and oogenesis. Studies suggest its involvement in meiotic progression, chromosomal stability, and RNA metabolism, potentially through interactions with spliceosomal components or chromatin-modifying enzymes. The recombinant form is typically produced in heterologous systems like *E. coli* or mammalian cells (e.g., HEK293), ensuring proper post-translational modifications for functional studies. Researchers utilize recombinant GSG1 to investigate fertility disorders, germ cell tumors, and epigenetic regulation mechanisms. Its phosphorylation-dependent activity and dynamic expression patterns during germ cell maturation make it a focus for understanding infertility and developmental abnormalities. Current applications also extend to generating antibodies for diagnostic tools and exploring its role in transposon silencing in germline genomes. Further studies aim to elucidate its molecular partners and signaling pathways, bridging gaps in reproductive medicine and genetic disease research.
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