| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSDML |
| Uniprot No | Q8TAX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-411aa |
| 氨基酸序列 | MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMDILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWSQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSAGLDIHFRGKTKSFPEGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISRELHMEDSDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSPPDMDYDPEARILCALYVVVSILLELAEGPTSVSS |
| 分子量 | 70.95 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GSDML蛋白的参考文献示例(注：文献信息基于模拟场景，可能存在虚构内容)：
1. **文献名称**：**"Expression and functional characterization of recombinant human GSDML in caspase-mediated pyroptosis"**
**作者**：Smith J. et al. (2020)
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组人GSDML蛋白，发现其N端结构域可在细胞膜上形成孔道，诱导焦亡;通过体外实验证实其激活依赖caspase-3/7.提示其在炎症反应中的作用。
2. **文献名称**：**"Structural basis of GSDML pore formation in lipid bilayers revealed by cryo-EM"**
**作者**：Lee C. et al. (2021)
**摘要**：利用冷冻电镜解析了重组GSDML蛋白的寡聚结构，揭示其β-折叠桶状孔道形成机制，并通过脂质体实验证明其对磷脂酰丝氨酸的选择性通透性。
3. **文献名称**：**"GSDML promotes tumor progression via NF-κB signaling in gastric cancer"**
**作者**：Wang Y. et al. (2019)
**摘要**：通过重组GSDML蛋白体外处理胃癌细胞，发现其通过激活NF-κB通路增强细胞迁移，提示GSDML可能作为癌症治疗的潜在靶点。
4. **文献名称**：**"Post-translational modification regulates GSDML-mediated pyroptosis in airway epithelium"**
**作者**：Zhang R. et al. (2022)
**摘要**：研究发现重组GSDML蛋白的磷酸化修饰(Ser58位点)抑制其孔道形成能力，可能为呼吸道炎症疾病提供调控机制。
**注**：GSDML的研究相对较少，部分文献可能与GSDMD或其他Gasdermin家族成员存在交叉。建议结合最新数据库(如UniProt、PubMed)核实。
Gasdermin-like protein (GSDML), a member of the gasdermin family, is a pore-forming protein implicated in regulated cell death and inflammatory responses. Unlike its well-studied homolog GSDMD, which plays a central role in pyroptosis via caspase-mediated cleavage, GSDML remains less characterized. It shares the conserved N-terminal pore-forming domain and C-terminal autoinhibitory domain typical of gasdermins, but exhibits distinct expression patterns and regulatory mechanisms.
GSDML is encoded within the human 17q21 locus, a region linked to inflammatory diseases and cancer susceptibility. It is predominantly expressed in epithelial tissues and immune cells, suggesting roles in mucosal immunity and barrier function. Studies associate GSDML with autoimmune disorders, chronic inflammation, and tumor progression, though its exact activation triggers remain unclear. Unlike other gasdermins, GSDML may interact with non-canonical proteases or undergo alternative post-translational modifications.
Recombinant human GSDML protein is typically produced in E. coli or mammalian expression systems for functional studies. Its applications include deciphering gasdermin family diversity, modeling pore-formation mechanisms, and screening therapeutic agents targeting inflammatory cell death pathways. Current research focuses on resolving its structure-function relationships and pathological relevance, particularly in cancers and autoimmune conditions where GSDML dysregulation is observed.
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