| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSC2 |
| Uniprot No | O15499 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 氨基酸序列 | MAAAAGGAASRRGAGRPCPFSIEHILSSLPERSLPARAACPPQPAGRQSPAKPEEPGAPEAAPCACCCCCGPRAAPCGPPEAAAGLGARLAWPLRLGPAVPLSLGAPAGGSGALPGAVGPGSQRRTRRHRTIFSEEQLQALEALFVQNQYPDVSTRERLAGRIRLREERVEVWFKNRRAKWRHQKRASASARLLPGVKKSPKGSC |
| 分子量 | 48.95 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant human GSC2 protein regulates neural crest cell differentiation" by Smith et al.**
摘要：研究报道了重组人GSC2蛋白在体外通过调控Wnt/β-catenin通路促进神经嵴细胞分化的机制。
2. **"Expression and purification of functional human GSC2 in a prokaryotic system" by Lee & Chen**
摘要：本文描述了一种利用大肠杆菌表达系统高效制备可溶性重组人GSC2蛋白的方法，并验证了其DNA结合活性。
3. **"GSC2-mediated transcriptional repression in cancer metastasis" by Gupta et al.**
摘要：研究发现重组人GSC2蛋白通过抑制上皮-间质转化(EMT)相关基因，抑制乳腺癌细胞的迁移和侵袭。
4. **"Structural analysis of human GSC2 reveals its role in craniofacial development" by Wang et al.**
摘要：通过X射线晶体学解析重组人GSC2蛋白结构，结合基因敲除实验揭示其在颅面发育中的关键作用。
(注：上述文献为模拟示例，实际引用时需核实真实存在的论文及细节。)
Recombinant human GSC2 (Goosecoid2) protein is a genetically engineered version of the naturally occurring transcription factor encoded by the *GSC2* gene in humans. GSC2 belongs to the homeobox protein family, characterized by a conserved DNA-binding domain known as the homeodomain. This protein plays a critical role in early embryonic development, particularly in establishing cell fate, tissue patterning, and organogenesis. It is involved in regulating genes that control the formation of structures such as the nervous system, skeleton, and craniofacial regions. Dysregulation of GSC2 has been linked to developmental disorders and certain cancers, highlighting its importance in both normal and pathological processes.
The recombinant form of GSC2 is produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling large-scale purification for research and therapeutic applications. This engineered protein retains the functional domains necessary for DNA binding and interaction with co-regulators, allowing scientists to study its molecular mechanisms in vitro or in cellular models. Researchers utilize recombinant GSC2 to investigate its role in signaling pathways (e.g., Wnt, BMP) and to explore its potential in regenerative medicine, disease modeling, or targeted therapies. Its application extends to drug screening platforms, where modulating GSC2 activity could offer insights into treating developmental anomalies or malignancies driven by its aberrant expression.
×
