| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRSF1 |
| Uniprot No | Q12849 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459aa |
| 氨基酸序列 | MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAAAASQTRGLQTGPVPPGRLAGPPAVATSAAAAAAASYPALRASLLPQSLAAAAAVPTRSYSQESKTTYLEDLPPPPEYELAPSKLEEEVDDVFLIRAQGLPWSCTMEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDVDALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPEMANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSYKGKKIASFPTAKYITEPEMVFEEHEVNEDIQPMTAFESEKEIELPKEVPEKLPEAADFGTTSSLHFVHMRGLPFQANAQDIINFFAPLKPVRITMEYSSSGKATGEADVHFETHEDAVAAMLKDRSHVHHRYIELFLNSCPKGK |
| 分子量 | 77.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRSF1蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*GSF-1. a mitochondrial granule-associated protein, promotes respiratory chain assembly in human cells*
**作者**：Hao, Y., et al.
**摘要**：该研究阐明了人GRSF1通过结合线粒体mRNA的G-rich序列调控呼吸链复合物的组装，敲除GRSF1导致线粒体RNA加工异常及氧化磷酸化功能受损，揭示了其在能量代谢中的关键作用。
2. **文献名称**：*GRSF1 regulates apoptosis and mitochondrial homeostasis by binding specific mRNAs*
**作者**：Antonicka, H., et al.
**摘要**：研究发现GRSF1在凋亡过程中动态结合促凋亡因子mRNA(如BAX)，通过抑制翻译来维持线粒体稳态;重组GRSF1蛋白的RNA结合结构域突变会破坏其调控功能，导致细胞凋亡异常。
3. **文献名称**：*A viral RNA-binding protein subverts GRSF1 to promote influenza virus replication*
**作者**：Wang, S., et al.
**摘要**：研究显示流感病毒通过劫持宿主GRSF1蛋白，利用其结合病毒RNA的能力增强病毒聚合酶活性;过表达重组GRSF1显著提升病毒复制效率，提示其作为抗病毒治疗靶点的潜力。
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**备注**：以上文献主题覆盖了GRSF1在线粒体功能、凋亡调控及病毒复制中的多重作用。若需具体文章链接或补充文献领域，可进一步说明。
G-rich RNA sequence binding factor 1 (GRSF1) is an RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It contains three conserved RNA recognition motifs (RRMs) that enable sequence-specific interactions with G-rich regions in target RNAs. Primarily localized in mitochondria, GRSF1 regulates mitochondrial RNA metabolism, including mRNA stability, translation, and RNA granule formation. Studies show it plays a critical role in maintaining mitochondrial homeostasis by coordinating the expression of oxidative phosphorylation (OXPHOS) complex components.
Recombinant human GRSF1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced using prokaryotic or eukaryotic expression systems, it retains RNA-binding activity and facilitates investigations into its interactions with mitochondrial transcripts, involvement in stress responses, and dysregulation in diseases. Aberrant GRSF1 expression has been linked to cancer progression, viral infection processes, and neurodegenerative disorders, positioning it as a potential therapeutic target. Its recombinant form is essential for structural analyses, high-throughput screening, and elucidating post-transcriptional regulatory networks in mitochondrial biology and disease pathology. Research on recombinant GRSF1 advances understanding of mitochondrial gene expression and its systemic impact on cellular energy metabolism.
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