| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRPEL2 |
| Uniprot No | Q8TAA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MAVRSLWAGRLRVQRLLAWSAAWESKGWPLPFSTATQRTAGEDCRSEDPPDELGPPLAERALRVKAVKLEKEVQDLTVRYQRAIADCENIRRRTQRCVEDAKIFGIQSFCKDLVEVADILEKTTECISEESEPEDQKLTLEKVFRGLLLLEAKLKSVFAKHGLEKLTPIGDKYDPHEHELICHVPAGVGVQPGTVALVRQDGYKLHGRTIRLARVEVAVESQRRL |
| 分子量 | 51.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GRPEL2蛋白的参考文献示例(部分信息为虚构合成，仅供参考)：
1. **文献名称**：**"GRPEL2 Regulates Mitochondrial Protein Import and Cellular Redox Homeostasis"**
**作者**：Smith, J., et al.
**摘要**：该研究阐明了重组人GRPEL2蛋白在线粒体蛋白质输入中的作用，证实其通过与mtHSP70结合促进前体蛋白的折叠和运输，并揭示其缺失会导致细胞氧化还原失衡。
2. **文献名称**：**"Recombinant Human GRPEL2 Enhances Stress Resilience in Neuronal Cells"**
**作者**：Wang, L., et al.
**摘要**：通过体外表达重组人GRPEL2.发现其可减轻神经细胞氧化应激损伤，机制涉及调控线粒体蛋白酶体活性和抑制凋亡信号通路。
3. **文献名称**：**"Structural and Functional Analysis of GRPEL2 in Cardiomyocyte Protection"**
**作者**：Chen, Y., et al.
**摘要**：利用重组GRPEL2蛋白进行结构解析和功能实验，证明其在心肌缺血再灌注损伤中通过维持线粒体膜电位和ATP合成发挥保护作用。
4. **文献名称**：**"GRPEL2 Knockdown Disrupts Mitochondrial Dynamics: Insights from Recombinant Protein Rescue Experiments"**
**作者**：Gupta, R., et al.
**摘要**：通过重组GRPEL2蛋白回补实验，验证了其在维持线粒体裂变-融合平衡中的必要性，并揭示了与帕金森病相关蛋白PINK1的潜在互作。
**备注**：以上文献为模拟内容，实际研究中请通过PubMed、Web of Science等平台使用关键词“GRPEL2”“recombinant GRPEL2”“mitochondrial chaperone”检索真实文献。
GrpE-like 2 (GRPEL2) is a mitochondrial nucleotide exchange factor that plays a critical role in regulating the mitochondrial heat shock protein 70 (HSP70) system, essential for protein homeostasis. As a member of the GrpE family, it facilitates the ATP/ADP cycle of HSP70 chaperones by promoting nucleotide exchange, thereby aiding in protein folding, import, and stress response within the mitochondria. Unlike its homolog GRPEL1. which is broadly expressed, GRPEL2 exhibits tissue-specific expression, with higher levels observed in testes and certain tumors, suggesting potential roles in germ cell development or cancer progression.
Recombinant human GRPEL2 protein is typically produced using bacterial or eukaryotic expression systems to study its structural and functional properties. Research focuses on its interaction with mitochondrial HSP70 (mtHSP70/HSPA9), its impact on protein aggregation diseases, and links to pathologies like neurodegenerative disorders and cancer. Dysregulation of GRPEL2 has been associated with impaired mitochondrial function, oxidative stress, and apoptosis. Its recombinant form enables biochemical assays, structural studies, and exploration of therapeutic strategies targeting mitochondrial proteostasis. Despite progress, the precise mechanisms underlying GRPEL2's tissue specificity and disease relevance remain under investigation.
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