| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRM8 |
| Uniprot No | O00222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-908aa |
| 氨基酸序列 | MVCEGKRSASCPCFFLLTAKFYWILTMMQRTHSQEYAHSIRVDGDIILGGLFPVHAKGERGVPCGELKKEKGIHRLEAMLYAIDQINKDPDLLSNITLGVRILDTCSRDTYALEQSLTFVQALIEKDASDVKCANGDPPIFTKPDKISGVIGAAASSVSIMVANILRLFKIPQISYASTAPELSDNTRYDFFSRVVPPDSYQAQAMVDIVTALGWNYVSTLASEGNYGESGVEAFTQISREIGGVCIAQSQKIPREPRPGEFEKIIKRLLETPNARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIAEGAVTILPKRASIDGFDRYFRSRTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKCTGLERIARDSSYEQEGKVQFVIDAVYSMAYALHNMHKDLCPGYIGLCPRMSTIDGKELLGYIRAVNFNGSAGTPVTFNENGDAPGRYDIFQYQITNKSTEYKVIGHWTNQLHLKVEDMQWAHREHTHPASVCSLPCKPGERKKTVKGVPCCWHCERCEGYNYQVDELSCELCPLDQRPNMNRTGCQLIPIIKLEWHSPWAVVPVFVAILGIIATTFVIVTFVRYNDTPIVRASGRELSYVLLTGIFLCYSITFLMIAAPDTIICSFRRVFLGLGMCFSYAALLTKTNRIHRIFEQGKKSVTAPKFISPASQLVITFSLISVQLLGVFVWFVVDPPHIIIDYGEQRTLDPEKARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIIWLAFIPIFFGTAQSAEKMYIQTTTLTVSMSLSASVSLGMLYMPKVYIIIFHPEQNVQKRKRSFKAVVTAATMQSKLIQKGNDRPNGEVKSELCESLETNTSSTKTTYISYSNHSI |
| 分子量 | 101.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人GRM8蛋白相关的参考文献示例(注：以下内容为模拟示例，具体文献需通过学术数据库验证):
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1. **《Functional expression and characterization of human metabotropic glutamate receptor subtype 8 (GRM8) in HEK293 cells》**
- **作者**: Johnson A, et al.
- **摘要**: 研究报道了在HEK293细胞中成功表达重组人GRM8蛋白，验证其通过抑制cAMP信号通路介导谷氨酸能神经传递的功能，并开发了基于细胞的高通量药物筛选平台。
2. **《Structural insights into GRM8 activation using cryo-EM》**
- **作者**: Zhang L, et al.
- **摘要**: 通过冷冻电镜技术解析重组人GRM8蛋白的三维结构，揭示其配体结合域和跨膜区的构象变化，为靶向GRM8的变构调节剂设计提供结构基础。
3. **《GRM8-dependent modulation of synaptic plasticity in rodent models of depression》**
- **作者**: Smith R, et al.
- **摘要**: 利用重组人GRM8蛋白探究其对突触可塑性的调控作用，发现GRM8激动剂可缓解抑郁模型动物的行为异常，提示其在抗抑郁治疗中的潜力。
4. **《Development of a GRM8-specific radioligand binding assay for pharmacological studies》**
- **作者**: Tanaka Y, et al.
- **摘要**: 基于重组人GRM8蛋白建立了放射性配体结合实验体系，系统评估了多种化合物对GRM8的亲和力，助力精神疾病相关药物研发。
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建议通过 **PubMed、Web of Science** 或 **Google Scholar** 查询具体文献。如需精确信息，可补充GRM8研究关键词(如“重组表达”“信号通路”“疾病模型”)进一步筛选。
The metabotropic glutamate receptor 8 (GRM8), encoded by the GRM8 gene, is a class C G protein-coupled receptor (GPCR) predominantly expressed in the central nervous system. It belongs to the group III metabotropic glutamate receptors (mGluRs), which inhibit neurotransmitter release by coupling to Gαi/o proteins upon activation by glutamate, the primary excitatory neurotransmitter. GRM8 plays a critical role in modulating synaptic plasticity, neural excitability, and cognitive functions, with implications in neuropsychiatric disorders such as anxiety, schizophrenia, and addiction.
Recombinant human GRM8 protein is engineered for in vitro studies to elucidate its structure, signaling mechanisms, and interactions with potential therapeutics. Produced using mammalian or insect cell expression systems, it often includes tags (e.g., FLAG, His-tag) for purification and detection. Research leveraging recombinant GRM8 focuses on screening small-molecule modulators, mapping ligand-binding domains, and characterizing receptor dynamics. Challenges include preserving its native conformation due to its large extracellular domain and complex transmembrane topology.
Current studies aim to develop selective agonists/antagonists targeting GRM8 to address neurological diseases, emphasizing its therapeutic potential. However, incomplete understanding of its splice variants and downstream effector pathways limits clinical translation. Recombinant GRM8 remains a vital tool for decoding glutamate signaling and advancing neuropharmacology.
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