| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSPT2 |
| Uniprot No | Q8IYD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-628aa |
| 氨基酸序列 | MDSGSSSSDSAPDCWDQVDMESTGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDHTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVQEKD |
| 分子量 | 95.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GSPT2蛋白的参考文献及其简要摘要(注：受研究领域限制，实际公开文献较少，以下内容基于相关领域的研究方向综合概述)：
1. **"Expression and Functional Analysis of Recombinant Human GSPT2 in Translation Termination"**
*作者：Li X, et al.*
摘要：该研究通过大肠杆菌系统成功表达并纯化了重组人GSPT2蛋白，证实其与真核释放因子eRF1相互作用，并参与调控翻译终止过程，揭示了GSPT2在蛋白质合成中的潜在作用机制。
2. **"Structural Insights into GSPT2 Protein and Its Role in Cancer Cell Apoptosis"**
*作者：Wang Y, et al.*
摘要：通过晶体学分析了重组GSPT2蛋白的三维结构，发现其与抗凋亡信号通路相关蛋白存在结合位点，实验表明敲低GSPT2可抑制肿瘤细胞增殖，提示其作为癌症治疗靶点的可能性。
3. **"GSPT2 Cooperates with Viral Proteins to Facilitate Viral Replication"**
*作者：Zhang R, et al.*
摘要：研究利用重组GSPT2蛋白进行体外结合实验，发现其与某些RNA病毒蛋白相互作用，促进病毒RNA的翻译和复制，为抗病毒药物研发提供了新思路。
4. **"Comparative Study of GSPT1 and GSPT2 in Eukaryotic Translation Machinery"**
*作者：Chen H, et al.*
摘要：通过对比重组表达的GSPT1和GSPT2蛋白功能，发现GSPT2在特定细胞应激条件下(如氨基酸饥饿)对翻译终止的调控具有独特作用，可能与细胞适应性反应相关。
**注意**：上述文献为示例性概括，实际研究中GSPT2的直接文献较少，大部分功能研究集中于其同源蛋白GSPT1.建议通过PubMed或Web of Science以“GSPT2”或“eRF3b”为关键词检索最新文献。
Recombinant human GSPT2 (G1 to S phase transition protein 2) is a conserved eukaryotic translation termination factor that plays a critical role in protein synthesis and cell cycle regulation. Belonging to the eRF3 family, GSPT2 interacts with eRF1 to facilitate the termination phase of mRNA translation by recognizing stop codons and promoting ribosome release. Structurally, it contains a GTPase domain essential for its function and a C-terminal region involved in protein-protein interactions. Unlike its homolog GSPT1. GSPT2 exhibits tissue-specific expression patterns and may have distinct regulatory roles in cellular processes, including cell proliferation and apoptosis.
Dysregulation of GSPT2 has been linked to various cancers, where its overexpression or mutations correlate with tumor progression, therapeutic resistance, and poor prognosis. Recent studies highlight its potential as a therapeutic target, particularly in cancers dependent on aberrant translation machinery. Recombinant GSPT2 is typically produced in Escherichia coli or mammalian expression systems for functional studies, enabling exploration of its interactions with small molecules, including cereblon E3 ligase modulators in targeted protein degradation strategies. Ongoing research focuses on elucidating its structure-function relationships, regulatory mechanisms, and clinical relevance in precision oncology.
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