关键信息
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基因名
ZCCHC12
- 应用
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别名
ZCCHC12；SIZN1；Zinc finger CCHC domain-containing Protein 12
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6PEW1
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表达区间
1-402aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMASIIAR VGNSRRLNAP LPPWAHSMLR SLGRSLGPIM ASMADRNMKL FSGRVVPAQG EETFENWLTQ VNGVLPDWNM SEEEKLKRLM KTLRGPAREV MRVLQATNPN LSVADFLRAM KLVFGESESS VTAHGKFFNT LQAQGEKASL YVIRLEVQLQ NAIQAGIIAE KDANRTRLQQ LLLGGELSRD LRLRLKDFLR MYANEQERLP NFLELIRMVR EEEDWDDAFI KRKRPKRSES MVERAVSPVA FQGSPPIVIG SADCNVIEID DTLDDSDEDV ILVESQDPPL PSWGAPPLRD RARPQDEVLV IDSPHNSRAQ FPSTSGGSGY KNNGPGEMRR ARKRKHTIRC SYCGEEGHSK ETCDNESDKA QVFENLIITL QELTHTEMER SRVAPGEYND FSEPL
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ZCCHC12 is a member of the zinc finger CCCH-type protein family, which plays a crucial role in various biological processes, including regulation of gene expression, RNA metabolism, and cellular stress responses. Research has shown that ZCCHC12 is involved in modulating the expression of genes associated with tumor growth and progression, making it a potential target for cancer therapy. Its unique structure, characterized by multiple zinc finger motifs, allows it to bind specific RNA molecules, influencing their stability and translation. Understanding the functional mechanisms of ZCCHC12 is essential, as it may provide insights into the underlying molecular pathways that contribute to diseases such as cancer. Additionally, studies have suggested that ZCCHC12 may have implications in other physiological processes such as inflammation and immune responses. Therefore, the recombinant expression and characterization of ZCCHC12 protein are vital steps toward elucidating its biological roles and therapeutic potential. Advanced techniques, including recombinant DNA technology and protein purification, are utilized to produce this protein in substantial quantities for functional assays, structural studies, and potential application in drug development. Thus, ZCCHC12 not only represents an interesting subject of study in molecular biology but also holds promise for the development of novel therapeutic strategies in cancer and other diseases.
