| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2H3 |
| Uniprot No | Q13889 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308aa |
| 氨基酸序列 | MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMTKSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNVIFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAKKKKLKVSA |
| 分子量 | 60.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTF2H3蛋白的3篇文献摘要示例(注：部分内容为模拟虚构，仅供参考，实际引用需核对真实文献)：
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1. **文献名称**: Structural Insights into Human GTF2H3 Within the TFIIH Complex
**作者**: Smith A et al.
**摘要**: 本研究通过体外重组表达人GTF2H3蛋白，结合冷冻电镜技术解析其与TFIIH复合物的相互作用，揭示了其在RNA聚合酶II介导的转录起始和DNA损伤修复中的关键构象变化。
2. **文献名称**: Functional Characterization of Recombinant GTF2H3 in Nucleotide Excision Repair
**作者**: Tanaka K et al.
**摘要**: 利用重组人GTF2H3蛋白进行体外修复实验，证明其通过与XPD蛋白的互作，在紫外线诱导的DNA损伤修复中不可或缺，并建立了突变体功能缺失与着色性干皮病的关联机制。
3. **文献名称**: CRISPR Screening Identifies GTF2H3 as a Vulnerability in Cancer Cells
**作者**: Wang L et al.
**摘要**: 结合重组GTF2H3功能回补实验，发现该蛋白缺失导致癌细胞转录-修复偶联通路异常，为靶向TFIIH的癌症治疗策略提供了新依据。
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如需查找真实文献，建议在 **PubMed** 或 **Web of Science** 中搜索关键词：**"GTF2H3 recombinant"**、**"TFIIH subunit expression"** 或 **"GTF2H3 structure function"**，并优先选择近5年内发表的综述或高影响力研究论文。
Recombinant human GTF2H3 protein is a key component of the general transcription factor IIH (TFIIH) complex, which plays dual roles in transcription initiation by RNA polymerase II and nucleotide excision repair (NER) of damaged DNA. GTF2H3. also known as p34. is one of ten subunits of TFIIH. Structurally, it contains a conserved zinc-binding domain critical for stabilizing interactions within the core TFIIH subcomplex. Functionally, TFIIH enables promoter DNA unwinding during transcription initiation and recruits repair factors to UV-induced DNA lesions or oxidative damage sites through NER pathways. Recombinant GTF2H3 is typically produced in prokaryotic or eukaryotic expression systems (e.g., E. coli, insect cells) using genetic engineering, often with affinity tags (e.g., His-tag) for purification. Its availability facilitates studies on TFIIH assembly, transcription-regulation mechanisms, and DNA repair processes, particularly transcription-coupled repair defects linked to diseases like xeroderma pigmentosum. Researchers also use recombinant GTF2H3 to investigate TFIIH-related mutations in cancer and developmental disorders, screen small-molecule inhibitors targeting transcription-repair interfaces, and analyze structural dynamics via biochemical or biophysical assays. Its biochemical reconstitution aids in dissecting functional contributions to gene expression fidelity and genome stability maintenance.
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