| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSX2 |
| Uniprot No | Q9BZM3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304aa |
| 氨基酸序列 | MSRSFYVDSLIIKDTSRPAPSLPEPHPGPDFFIPLGMPPPLVMSVSGPGCPSRKSGAFCVCPLCVTSHLHSSRGSVGAGSGGAGAGVTGAGGSGVAGAAGALPLLKSQFSSAPGDAQFCPRVNHAHHHHHPPQHHHHHHQPQQPGSAAAAAAAAAAAAAAAALGHPQHHAPVCTATTYNVADPRRFHCLTMGGSDASQVPNGKRMRTAFTSTQLLELEREFSSNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKGTQRNSHAGCKCVGSQVHYARSEDEDSLSPASANDDKEISPL |
| 分子量 | 33.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GSX2蛋白的3篇参考文献概览(基于模拟检索和文献内容推断)：
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1. **标题**：*GSX2 regulates oligodendrocyte differentiation in the developing spinal cord*
**作者**：S. Mizuguchi et al.
**摘要**：研究揭示了重组人GSX2蛋白在小鼠脊髓发育中通过调控特定转录因子(如Olig2)促进少突胶质前体细胞分化的机制。通过体外实验验证了GSX2对髓鞘形成相关基因的激活作用。
2. **标题**：*In vitro generation of ventralized neural progenitors using recombinant human GSX2 protein*
**作者**：R. Zhang & L. Studer
**摘要**：利用重组人GSX2蛋白处理人多能干细胞，证明其可诱导腹侧前脑神经前体细胞的特化，为疾病建模(如帕金森病)提供了一种高效的细胞分化策略。
3. **标题**：*Structural and functional analysis of GSX2 in neural crest development*
**作者**：K. Watanabe et al.
**摘要**：通过重组表达纯化GSX2蛋白，结合X射线晶体学解析其DNA结合结构域的结构，揭示其通过结合特定靶基因启动子调控神经嵴细胞迁移的分子机制。
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**说明**：以上文献为模拟示例，实际检索建议通过PubMed、Web of Science等平台以关键词“recombinant GSX2”“human GSX2 protein”查找最新研究。部分功能研究可能间接涉及重组蛋白应用，需结合实验细节筛选。
Recombinant human GSX2 protein is a genetically engineered form of the transcription factor GSX2 (Genomic screened homeobox 2), also known as Gsh-2. which belongs to the homeobox gene family. GSX2 plays critical roles during embryonic development, particularly in the central nervous system. It is essential for patterning the dorsal-ventral axis of the neural tube and regulates the specification of neural progenitor cells, influencing the differentiation of specific neuronal subtypes, including inhibitory interneurons and dopaminergic neurons. GSX2 functions by binding to DNA and modulating the expression of downstream target genes, such as Pax6 and Ngn2. which are vital for neurogenesis. Unlike its homolog GSX1. GSX2 shows a more restricted expression pattern, primarily in the ventral telencephalon and spinal cord.
The recombinant form is produced using expression systems (e.g., E. coli, mammalian cells) to enable functional studies and therapeutic research. Its applications span stem cell biology, neurodevelopmental studies, and disease modeling, particularly for neurological disorders like Parkinson’s disease, where dopaminergic neuron loss is implicated. Researchers utilize recombinant GSX2 to explore its potential in guiding stem cell differentiation or repairing neural circuits. Additionally, it serves as a tool to dissect molecular mechanisms underlying neural patterning and transcriptional regulation. Studies on GSX2 also contribute to understanding congenital defects linked to homeobox gene mutations. The protein’s stability and activity are rigorously validated to ensure experimental reproducibility in both in vitro and in vivo settings.
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