| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSTCD |
| Uniprot No | Q8NEC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-632aa |
| 氨基酸序列 | MKAIKKSLTEEEYLYLDFSHQTEGCIFPLHTSVTLFLLSYCDCKIFKICLVVTKEVSRDSSLLRDDLIQDVEIQIISRQELPPIVQNCCLPAVVERSDNFCRAGLAVVLRHIIQKSYEADPLKKELLELLGFKKTCLKACAEVSQWTRLCELTIPLAIENFLRESSDQPPTIPVEILQLEKKLSEPVRVHNDDKLRRQKLKQQKADGVGPPLTKGKAKSKVHTQETSEGLDSSSKSLELKVAFSKLTVQEEPATTNREPSHIRKAKASDLPPLEHVFAEGLYFTLADIVLLPCIHHFLVIISRKFSEKLVEFPLLASWYQRIQEVPGVKTASKCGIQFLHLPKLLTTSTEQHPNLCEVPGVEEQSDPLFIGGPRPTMAKLMEKGIEVMFSPHPCPTWTLDWNVLPAAVSPKEGKMSSDRALRKQQQLNNLVYVVTNQAKPGDRIVDFCSGGGHVGIVLAHMLPSCQVTLIENKELSLIRAKKRSDELGLSNIWFIQANMEYFTGMFNIGVALHACGVATDMVIEHCIKTRASFVTCPCCYGFIQNTSKFNFPKSEQFKKTLSYKEHMILCRFADQTAVQLPPQRRLIGKQCMCLVDLDRARAAEECGYSVQVISMEPESCSPKNNMIVGVPI |
| 分子量 | 97.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GSTCD蛋白的示例参考文献(虚构示例，仅供格式参考)：
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1. **"Functional Characterization of Recombinant Human GSTCD Protein in Airway Epithelial Cells"**
*Johnson A. et al. (2018)*
该研究通过体外重组技术表达了人源GSTCD蛋白，验证其在气道上皮细胞中调节炎症因子表达的作用，并发现其可能通过TGF-β通路影响气道重塑。
2. **"Structural Insights into GSTCD and Its Role in Asthma Pathogenesis"**
*Lee S. & Martinez B. (2020)*
作者解析了重组人GSTCD蛋白的晶体结构，揭示了其与谷胱甘肽结合域的相互作用，并提出其基因变异可能通过影响蛋白稳定性参与哮喘易感性。
3. **"GSTCD Expression and Interaction with MYO1C in COPD Progression"**
*Wang Q. et al. (2019)*
研究利用重组GSTCD蛋白进行pull-down实验，发现其与MYO1C蛋白的互作可能影响慢性阻塞性肺病(COPD)患者的细胞骨架调节和黏液分泌。
4. **"Development of a GSTCD Knockout Model Using Recombinant Protein-Based Screening"**
*Brown T. et al. (2021)*
通过重组GSTCD蛋白制备抗体，构建了GSTCD基因敲除小鼠模型，发现其缺失导致肺功能下降和氧化应激反应增强。
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**注意**：以上文献及摘要为模拟示例，实际研究中需通过PubMed、Google Scholar等平台检索真实文献(关键词如 *"GSTCD recombinant protein"* 或 *"GSTCD functional study"*)。
Glutathione S-transferase C-terminal domain-containing protein (GSTCD) is a human protein encoded by the GSTCD gene, located on chromosome 4. It is characterized by a conserved glutathione S-transferase (GST) C-terminal domain, though its enzymatic activity remains unclear compared to canonical GSTs involved in detoxification and oxidative stress responses. GSTCD has drawn attention primarily for its potential role in respiratory health, with genome-wide association studies linking its genetic variants to lung function parameters, chronic obstructive pulmonary disease (COPD), and asthma.
Recombinant human GSTCD protein is engineered via heterologous expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties and molecular interactions. Its production typically involves cloning the GSTCD coding sequence into expression vectors, followed by purification using affinity chromatography. Researchers utilize this recombinant protein to explore its putative functions, such as involvement in airway smooth muscle regulation, extracellular matrix remodeling, or cellular signaling pathways relevant to pulmonary diseases.
Current studies focus on clarifying GSTCD’s mechanistic role in lung pathophysiology and its interplay with other disease-associated proteins like INTS12 or TGF-β pathway components. The protein’s structural features and interactome are under investigation to identify therapeutic targets for respiratory disorders. Recombinant GSTCD also serves as an antigen for antibody development, aiding in cellular localization and functional studies. Despite progress, its precise biological role remains an active area of respiratory research.
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