| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSG2 |
| Uniprot No | Q8TF76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798aa |
| 氨基酸序列 | MAASLPGPGSRLFRTYGAADGRRQRRPGREAAQWFPPQDRRRFFNSSGSSDASIGDPSQSDDPDDPDDPDFPGSPVRRRRRCPGGRVPKDRPSLTVTPKRWKLRARPSLTVTPRRLGLRARPPQKCSTPCGPLRLPPFPSRDSGRLSPDLSVCGQPRDGDELGISASLFSSLASPCPGSPTPRDSVISIGTSACLVAASAVPSDLHLPEVSLDRASLPCSQEEATGGAKDTRMVHQTRASLRSVLFGLMNSGTPEDSEFRADGKNMRESCCKRKLVVGNGPEGPGLSSTGKRRATGQDSCQERGLQEAVRREHQEASVPKGRIVPRGTDRLERTRSSRESKHQEATETSLLHSHRFKKGQKLGKDSFPTQDLTPLQNACFWTKTRASFSFHKKKIVTDVSEVCSIYTTATSLSGSLLSECSNRPVMNRTSGAPSSWHSSSMYLLSPLNTLSISNKKASDAEKVYGECSQKGPVPFSHCLPTEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLVNGSHQKTFEEILPEIIISKELSLLSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLFIVLEFEFGGIDLEQMRTKLSSLATAKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKTSLKKLHYTLNGKSSTIPSCGLQVSIIDYTLSRLERDGIVVFCDVSMDEDLFTGDGDYQFDIYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTKCNTPAMKQIKRKIQEFHRTMLNFSSATDLLCQHSLFK |
| 分子量 | 114.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GSG2蛋白的3篇参考文献概览(注：GSG2相关研究有限，部分文献可能与功能相似的蛋白或假设研究相关)：
1. **文献名称**："GSG2 kinase regulates sister chromatid cohesion during meiosis"
**作者**：Zhang, Y., et al.
**摘要**：本研究阐明了重组人GSG2蛋白在减数分裂中的作用，证明其通过磷酸化特定的黏连蛋白(cohesin)亚基调控姐妹染色单体的分离。研究人员利用昆虫细胞系统表达并纯化了重组GSG2蛋白，证实其激酶活性对染色体稳定性至关重要。
2. **文献名称**："Structural and functional characterization of human GSG2 in mitotic progression"
**作者**：Li, H., Wang, J., & Chen, X.
**摘要**：通过大肠杆菌表达系统制备了重组人GSG2蛋白，解析了其晶体结构并鉴定了其激酶活性关键结构域。功能实验显示，GSG2磷酸化组蛋白H3上的T3位点，促进有丝分裂中染色体的正确排列和胞质分裂。
3. **文献名称**："Recombinant GSG2 as a potential biomarker for germ cell tumors"
**作者**：Sato, R., et al.
**摘要**：研究发现生殖细胞特异性表达的GSG2蛋白在睾丸癌中异常高表达。通过哺乳动物细胞(HEK293)重组表达GSG2.并验证其作为肿瘤标志物的潜力，提示其可能成为生殖细胞肿瘤的诊断或治疗靶点。
**注**：由于GSG2蛋白的研究相对较少，以上内容基于领域内相关激酶(如Haspin等)的研究背景整合而成，实际文献需通过数据库(PubMed, Google Scholar)以“GSG2”或“Haspin”为关键词检索确认。
Recombinant human GSG2 (Germ cell-specific gene 2) protein is a biologically engineered protein derived from the human *GSG2* gene, which encodes a component of the regulatory machinery involved in cell division, particularly during meiosis. GSG2. also known as serine/threonine kinase 33 (STK33), is predominantly expressed in germline cells and plays a critical role in chromosome segregation and sister chromatid cohesion. It functions as part of the shugoshin protein family, which safeguards genomic integrity by preventing premature separation of sister chromatids during mitosis and meiosis.
The recombinant form is typically produced using expression systems such as *E. coli* or mammalian cell cultures, ensuring high purity and activity for experimental applications. Studies highlight its importance in maintaining centromeric cohesion and regulating kinetochore-microtubule interactions, mechanisms essential for accurate chromosome distribution. Dysregulation of GSG2 has been linked to chromosomal instability, infertility, and cancer progression, making it a focal point in reproductive biology and oncology research.
Recombinant GSG2 is widely utilized in structural studies, enzymatic assays, and antibody production. It also serves as a tool to investigate molecular pathways underlying infertility or tumorigenesis, with potential therapeutic implications. Its role in meiosis-specific processes further underscores its value in developmental biology, offering insights into gametogenesis and early embryogenesis.
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