| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2H4 |
| Uniprot No | Q92759 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MESTPSRGLNRVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVSGAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS |
| 分子量 | 76.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人GTF2H4蛋白相关的参考文献模板(注：部分内容为示例性描述，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Structural and Functional Analysis of Recombinant Human GTF2H4 in DNA Repair*
**作者**: Müller R. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人GTF2H4蛋白，通过晶体学解析其三维结构，发现其核心结构域对TFIIH复合体的稳定性至关重要。实验验证了GTF2H4在紫外线诱导的DNA损伤修复中的直接作用。
2. **文献名称**: *GTF2H4 Mutants Disrupt Transcriptional Regulation in Cancer Cells*
**作者**: Chen L., Wang Y.
**摘要**: 文章报道了重组人GTF2H4蛋白的功能缺失突变体在肺癌细胞模型中的应用，发现其突变会削弱RNA聚合酶II的转录延伸能力，并揭示其与癌症耐药性相关的分子机制。
3. **文献名称**: *Expression and Purification of Human GTF2H4 for Interaction Studies*
**作者**: Tanaka S. et al.
**摘要**: 开发了一种基于杆状病毒表达系统的重组GTF2H4蛋白高效制备方法，结合pull-down实验证实其与XPB蛋白(ERCC3)的相互作用，为研究TFIIH复合体组装提供技术基础。
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若需具体文献，建议在 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Web of Science** 中检索关键词 "recombinant human GTF2H4" 或 "GTF2H4 protein expression" 获取最新研究。
Recombinant human GTF2H4 protein is a key component of the general transcription factor IIH (TFIIH) complex, which plays essential roles in RNA polymerase II-mediated transcription initiation and nucleotide excision repair (NER) of DNA damage. GTF2H4 (General Transcription Factor IIH Subunit 4), encoded by the GTF2H4 gene, forms part of the core subcomplex of TFIIH alongside other subunits such as XPB and XPD. This protein is critical for stabilizing the interaction between TFIIH and promoter-bound RNA polymerase II, ensuring proper assembly of the pre-initiation complex during transcription. Additionally, it contributes to DNA repair by participating in the recognition and excision of damaged DNA segments through NER pathways.
The recombinant form of GTF2H4 is typically produced using engineered expression systems, such as *E. coli* or mammalian cell cultures, to enable studies on its structural and functional properties. Researchers utilize recombinant GTF2H4 to investigate molecular mechanisms underlying transcription regulation, DNA repair disorders (e.g., xeroderma pigmentosum), and TFIIH-related diseases. Its role in linking transcriptional defects to genetic instability has also made it a focus in cancer research and therapeutics development. Purified recombinant GTF2H4 is essential for *in vitro* assays, structural analyses (e.g., X-ray crystallography), and screening for molecules targeting TFIIH-associated processes. Studies on this protein enhance understanding of cellular responses to DNA damage and transcription-coupled repair pathways.
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