| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF3C2 |
| Uniprot No | Q8WUA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-911aa |
| 氨基酸序列 | MDTCGVGYVALGEAGPVGNMTVVDSPGQEVLNQLDVKTSSEMTSAEASVEMSLPTPLPGFEDSPDQRRLPPEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMPKKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAAQVALLYLQELAEELSTALPAPVSCPEGPKVSSPTKPKKIRQPAACPGGEEVDGAPRDEDFFLQVEAEDVEESEGPSESSSEPEPVVPRSTPRGSTSGKQKPHCRGMAPNGLPDHIMAPVWKCLHLTKDFREQKHSYWEFAEWIPLAWKWHLLSELEAAPYLPQEEKSPLFSVQREGLPEDGTLYRINRFSSITAHPERWDVSFFTGGPLWALDWCPVPEGAGASQYVALFSSPDMNETHPLSQLHSGPGLLQLWGLGTLQQESCPGNRAHFVYGIACDNGCIWDLKFCPSGAWELPGTPRKAPLLPRLGLLALACSDGKVLLFSLPHPEALLAQQPPDAVKPAIYKVQCVATLQVGSMQATDPSECGQCLSLAWMPTRPHQHLAAGYYNGMVVFWNLPTNSPLQRIRLSDGSLKLYPFQCFLAHDQAVRTLQWCKANSHFLVSAGSDRKIKFWDLRRPYEPINSIKRFLSTELAWLLPYNGVTVAQDNCYASYGLCGIHYIDAGYLGFKAYFTAPRKGTVWSLSGSDWLGTIAAGDISGELIAAILPDMALNPINVKRPVERRFPIYKADLIPYQDSPEGPDHSSASSGVPNPPKARTYTETVNHHYLLFQDTDLGSFHDLLRREPMLRMQEGEGHSQLCLDRLQLEAIHKVRFSPNLDSYGWLVSGGQSGLVRIHFVRGLASPLGHRMQLESRAHFNAMFQPSSPTRRPGFSPTSHRLLPTP |
| 分子量 | 125.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GTF3C2蛋白的示例参考文献(文献信息为假设，实际文献请通过学术数据库检索补充)：
1. **文献名称**：Structural and functional analysis of human GTF3C2 in RNA polymerase III transcription complex
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了重组人GTF3C2蛋白的结构，发现其锌指结构域对RNA聚合酶III招募至基因启动子具有关键作用，并验证了其在tRNA转录中的调控功能。
2. **文献名称**：GTF3C2 interacts with BRCA1 and modulates DNA damage response pathways
**作者**：Li X, et al.
**摘要**：研究发现重组人GTF3C2蛋白可与BRCA1蛋白结合，并通过ChIP-seq证实其在DNA损伤修复中的潜在角色，提示GTF3C2可能参与癌症相关通路。
3. **文献名称**：Development of a GTF3C2 knockout cell model to study neuronal development defects
**作者**：Wang Y, et al.
**摘要**：利用CRISPR技术构建GTF3C2敲除细胞系，发现其缺失导致神经元分化异常，推测GTF3C2通过调控非编码RNA影响神经发育疾病。
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建议通过 **PubMed/Google Scholar** 检索关键词：
`"recombinant human GTF3C2"` 或 `"GTF3C2 protein structure/function"`
可筛选近年文献关注其分子机制、疾病关联或技术应用(如纯化方法、互作蛋白筛选等)。
**Background of Recombinant Human GTF3C2 Protein**
Recombinant human GTF3C2 (General Transcription Factor 3C subunit 2) is a protein component of the RNA polymerase III (Pol III) transcription factor complex, which is essential for the synthesis of small non-coding RNAs, including tRNAs, 5S rRNA, and other regulatory RNAs. GTF3C2. along with other GTF3C subunits (GTF3C1. GTF3C3-6), forms a critical scaffold for RNA Pol III recruitment to target gene promoters.
GTF3C2 plays a role in stabilizing the TFIIIB-Pol III interaction, facilitating transcription initiation. It is ubiquitously expressed and localized in the nucleus, highlighting its fundamental role in cellular processes such as ribosome biogenesis, protein synthesis, and cell proliferation. Dysregulation of RNA Pol III activity, potentially linked to GTF3C2 dysfunction, has been implicated in cancers, neurodegenerative disorders, and viral infections.
Recombinant GTF3C2 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Its purified form allows researchers to investigate transcriptional mechanisms, protein-protein interactions, and disease-related mutations. Recent studies also explore its potential as a therapeutic target, particularly in cancers where RNA Pol III activity is elevated. Continued research on GTF3C2 enhances understanding of transcriptional regulation and its implications in human health and disease.
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