| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF3C3 |
| Uniprot No | Q9Y5Q9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-886aa |
| 氨基酸序列 | MSGFSPELIDYLEGKISFEEFERRREERKTREKKSLQEKGKLSAEENPDDSEVPSSSGINSTKSQDKDVNEGETSDGVRKSVHKVFASMLGENEDDEEEEEEEEEEEEEEETPEQPTAGDVFVLEMVLNRETKKMMKEKRPRSKLPRALRGLMGEANIRFARGEREEAILMCMEIIRQAPLAYEPFSTLAMIYEDQGDMEKSLQFELIAAHLNPSDTEEWVRLAEMSLEQDNIKQAIFCYTKALKYEPTNVRYLWERSSLYEQMGDHKMAMDGYRRILNLLSPSDGERFMQLARDMAKSYYEANDVTSAINIIDEAFSKHQGLVSMEDVNIAAELYISNKQYDKALEIITDFSGIVLEKKTSEEGTSEENKAPENVTCTIPDGVPIDITVKLMVCLVHLNILEPLNPLLTTLVEQNPEDMGDLYLDVAEAFLDVGEYNSALPLLSALVCSERYNLAVVWLRHAECLKALGYMERAAESYGKVVDLAPLHLDARISLSTLQQQLGQPEKALEALEPMYDPDTLAQDANAAQQELKLLLHRSTLLFSQGKMYGYVDTLLTMLAMLLKVAMNRAQVCLISSSKSGERHLYLIKVSRDKISDSNDQESANCDAKAIFAVLTSVLTKDDWWNLLLKAIYSLCDLSRFQEAELLVDSSLEYYSFYDDRQKRKELEYFGLSAAILDKNFRKAYNYIRIMVMENVNKPQLWNIFNQVTMHSQDVRHHRFCLRLMLKNPENHALCVLNGHNAFVSGSFKHALGQYVQAFRTHPDEPLYSFCIGLTFIHMASQKYVLRRHALIVQGFSFLNRYLSLRGPCQESFYNLGRGLHQLGLIHLAIHYYQKALELPPLVVEGIELDQLDLRRDIAYNLSLIYQSSGNTGMAQTLLYTYCSI |
| 分子量 | 127.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTF3C3蛋白的参考文献示例(注：以下内容为虚构，仅供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant Human GTF3C3 in Escherichia coli*
**作者**：Chen et al.
**摘要**：本研究报道了一种高效在大肠杆菌中表达和纯化重组人GTF3C3蛋白的方法。通过优化密码子使用和诱导条件，成功获得高纯度蛋白，并验证了其与RNA聚合酶III核心复合物的体外结合能力，为后续功能研究奠定基础。
2. **文献名称**：*Structural Insights into GTF3C3 and Its Role in RNA Polymerase III Transcription Initiation*
**作者**：Smith et al.
**摘要**：通过X射线晶体学解析了重组人GTF3C3蛋白的晶体结构，揭示了其与DNA结合的关键结构域。研究提出GTF3C3通过稳定启动子区域构象，促进RNA聚合酶III的转录起始复合物组装。
3. **文献名称**：*GTF3C3 Depletion Alters tRNA Synthesis and Cancer Cell Proliferation*
**作者**：Wang et al.
**摘要**：利用CRISPR技术敲低GTF3C3.发现其缺失导致细胞中tRNA水平显著下降，抑制癌细胞增殖。研究证实GTF3C3通过调控RNA聚合酶III依赖性转录，影响肿瘤发生通路。
4. **文献名称**：*A High-Throughput Screen for GTF3C3-Interacting Proteins Using Recombinant TAP-Tagged GTF3C3*
**作者**：Rodriguez et al.
**摘要**：构建带有串联亲和纯化标签(TAP)的重组人GTF3C3蛋白，通过质谱分析鉴定了多个新型相互作用蛋白，包括染色质修饰因子，提示其在表观遗传调控中的潜在作用。
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以上内容基于科学假设构造，如需真实文献，建议通过PubMed或Web of Science以“GTF3C3”、“recombinant GTF3C3”等关键词检索。
General transcription factor 3C subunit 3 (GTF3C3), also known as TFIIIC90. is a critical component of the RNA polymerase III (Pol III) transcription machinery. It is part of the TFIIIC complex, which directly binds promoter regions of small non-coding RNAs, including tRNA, 5S rRNA, and other regulatory RNAs, facilitating their transcription. GTF3C3 plays a structural and regulatory role in coordinating TFIIIC assembly and recruiting Pol III to target genes. Structurally, it contains conserved domains for protein-protein interactions and potential DNA-binding motifs, contributing to promoter recognition and complex stabilization.
Recombinant human GTF3C3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It enables researchers to investigate GTF3C3's role in transcription initiation, its interaction with other TFIIIC subunits (e.g., GTF3C1. GTF3C2), and its potential dysregulation in diseases. Aberrant Pol III activity linked to GTF3C3 has been implicated in cancers, viral infections, and neurodevelopmental disorders. Recombinant variants, often tagged for purification, are essential tools for *in vitro* binding assays, structural studies, and screening therapeutic agents targeting transcription abnormalities. Current research also explores its non-canonical roles in cell cycle regulation and chromatin remodeling, broadening its relevance in molecular biology.
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