| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEATR2 |
| Uniprot No | Q86Y56 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MRLKLFSILSTVLLRATDTINSQGQFPSYLETVTKDILAPNLQWHAGRTAAAIRTAAVSCLWALTSSEVLSAEQIRDVQETLMPQVLTTLEEDSKMTRLISCRIINTFLKTSGGMTDPEKLIKIYPELLKRLDDVSNDVRMAAASTLVTWLQCVKGANAKSYYQSSVQYLYRELLVHLDDPERAIQDAILDVPGSPSPSAMIGSFLRPPHPWRTVSQLNLFSS |
| 分子量 | 51.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HEATR2蛋白的参考文献示例(内容虚构，仅作示例参考)：
1. **文献名称**：**《HEATR2 interacts with CEP290 to regulate ciliogenesis through microtubule stability》**
**作者**：Smith A, et al.
**摘要**：研究利用重组HEATR2蛋白揭示其与CEP290的相互作用，证明其在纤毛形成中通过稳定微管结构促进纤毛组装，缺失导致纤毛相关疾病。
2. **文献名称**：**《Expression and functional characterization of HEATR2 mutants in primary ciliary dyskinesia》**
**作者**：Wang L, et al.
**摘要**：通过重组表达HEATR2突变体蛋白，发现其错误定位破坏纤毛内运输(IFT)，阐明HEATR2基因突变导致运动纤毛功能障碍的分子机制。
3. **文献名称**：**《A high-yield method for recombinant human HEATR2 production in mammalian cells》**
**作者**：Gupta R, et al.
**摘要**：开发了一种基于HEK293细胞系统的重组HEATR2蛋白高效表达与纯化方案，并验证其在体外结合实验中的活性，为功能研究提供工具。
4. **文献名称**：**《Structural insights into HEATR2’s role in the human cohesin complex assembly》**
**作者**：Tanaka K, et al.
**摘要**：结合冷冻电镜分析与重组HEATR2蛋白，揭示其通过C端结构域介导黏连蛋白(cohesin)复合体的组装，影响染色体分离过程。
(注：上述文献为示例性虚构内容，实际研究中建议通过PubMed或Google Scholar等平台检索真实文献。)
Human HEATR2 (HEAT repeat-containing protein 2) is a conserved eukaryotic protein characterized by multiple HEAT-repeat motifs, which mediate protein-protein interactions and structural scaffolding. It plays critical roles in cellular processes, including ciliogenesis, cell cycle regulation, and DNA damage response. Studies link HEATR2 to the assembly and stability of primary cilia—a sensory organelle essential for signaling pathways like Hedgehog. Dysfunctional HEATR2 is implicated in ciliopathies, particularly primary ciliary dyskinesia (PCD) with severe reproductive and respiratory complications. Mutations in the HEATR2 gene are associated with defective axonemal structure in motile cilia, leading to impaired mucociliary clearance and male infertility due to sperm flagellum abnormalities.
Recombinant human HEATR2 protein is engineered for functional and structural studies, typically produced in bacterial or mammalian expression systems with tags (e.g., His-tag) for purification. Its recombinant form enables in vitro analyses, such as interaction mapping with ciliary components (e.g., IFT complexes), enzymatic assays, and antibody development. Researchers also utilize it to explore disease mechanisms or screen therapeutic compounds targeting ciliopathies. Despite being less studied than other ciliary proteins, HEATR2’s involvement in both ciliogenesis and genomic stability highlights its dual regulatory significance. Current work focuses on resolving its 3D structure, post-translational modifications, and precise mechanistic contributions to cellular homeostasis, offering potential pathways for diagnostics or gene therapy in ciliary disorders.
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