| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H4 |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | SGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG |
| 分子量 | 11.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST2H4蛋白的参考文献示例(文献内容为模拟创作，仅供参考)：
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1. **标题**：*Expression and purification of recombinant human HIST2H4 for chromatin assembly studies*
**作者**：Zhang et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效表达重组人HIST2H4蛋白，优化了纯化条件以获得高纯度蛋白，验证其与H3组蛋白结合形成核小体核心的能力，为体外染色质重建实验提供材料基础。
2. **标题**：*HIST2H4 post-translational modifications in epigenetic regulation*
**作者**：Lee & Kim
**摘要**：通过质谱分析重组HIST2H4蛋白的乙酰化、甲基化修饰位点，发现其K20位点的甲基化与DNA损伤修复相关，修饰后的蛋白显著影响染色质结构和基因沉默过程。
3. **标题**：*Functional characterization of HIST2H4 mutations in cancer cell models*
**作者**：Garcia-Rodriguez et al.
**摘要**：利用重组HIST2H4蛋白突变体(如R36C)研究其对核小体稳定性的影响，发现该突变导致染色质异常凝聚并与乳腺癌细胞增殖异常相关。
4. **标题**：*Development of a HIST2H4-based peptide inhibitor for viral replication suppression*
**作者**：Wang et al.
**摘要**：基于重组HIST2H4蛋白N端结构设计多肽抑制剂，证明其通过竞争性结合病毒整合酶阻断HIV-1在宿主基因组中的整合，为抗病毒治疗提供新策略。
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**注**：上述文献为示例性质，实际研究需查询真实数据库(如PubMed)。建议使用关键词“recombinant HIST2H4”、“human histone H4”等结合具体研究方向筛选文献。
The human HIST2H4 protein, encoded by the HIST2H4 gene, is a member of the histone H4 family, which plays a fundamental role in chromatin structure and epigenetic regulation. Histones are core components of nucleosomes, where DNA wraps around histone octamers (two each of H2A, H2B, H3. and H4) to compact genetic material and regulate DNA accessibility. HIST2H4 is one of several H4 isoforms, differing from HIST1H4 variants by its chromosomal location and expression patterns. Recombination-derived HIST2H4 (recombinant HIST2H4) is typically produced in vitro using expression systems like *E. coli* or mammalian cells, enabling controlled studies of its biochemical properties. This protein retains conserved post-translational modification sites, such as lysine residues subject to acetylation, methylation, or ubiquitination, which influence chromatin remodeling and gene expression. Researchers use recombinant HIST2H4 to investigate nucleosome assembly, histone-DNA interactions, and epigenetic mechanisms in diseases like cancer, where histone dysregulation is common. It also serves as a tool to study inhibitors of histone-modifying enzymes for therapeutic development. Unlike native histones, recombinant versions allow standardized, contamination-free experimentation, making them critical for structural studies (e.g., X-ray crystallography) and functional assays. Overall, HIST2H4 exemplifies the intersection of chromatin biology and recombinant protein technology in advancing epigenetic research.
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