| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H4A |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | SGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG |
| 分子量 | 11.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST2H4A蛋白的参考文献示例(注：内容为模拟，建议通过学术数据库验证具体文献)：
1. **文献名称**：*"Expression and Functional Analysis of Recombinant Human HIST2H4A in Chromatin Remodeling"*
**作者**：M. Tanaka et al.
**摘要**：本研究报道了在大肠杆菌中高效表达并纯化重组人HIST2H4A蛋白的方法，验证了其与DNA结合的能力及在体外核小体组装实验中的作用，为染色质动力学研究提供了工具。
2. **文献名称**：*"HIST2H4A Lysine Acetylation Modulates Gene Regulation in vitro"*
**作者**：S. Patel & R. Kumar
**摘要**：通过重组HIST2H4A蛋白探究了特异性赖氨酸位点的乙酰化修饰对染色质结构的影响，发现其乙酰化可通过改变组蛋白-DNA相互作用参与基因沉默过程。
3. **文献名称**：*"Recombinant HIST2H4A as a Biomarker for Epigenetic Drug Screening"*
**作者**：L. Fernández et al.
**摘要**：开发了基于重组HIST2H4A的高通量筛选平台，用于评估靶向组蛋白修饰的抗癌化合物，证明其在表观遗传药物开发中的潜在应用价值。
4. **文献名称**：*"Structural Insights into HIST2H4A Mutations Associated with Developmental Disorders"*
**作者**：G. Wu et al.
**摘要**：利用重组HIST2H4A蛋白解析了致病性突变体的晶体结构，揭示了突变如何破坏组蛋白二聚化界面，进而导致染色质异常和神经发育缺陷。
建议通过PubMed或Google Scholar搜索**"recombinant HIST2H4A"**或**"human histone H4 expression"**获取真实文献。
The human HIST2H4A gene encodes histone H4. a core component of nucleosomes responsible for compacting DNA into chromatin. As part of the histone H4 family, HIST2H4A contributes to the structural stability of chromatin and regulates DNA accessibility for processes like transcription, replication, and repair. Histone H4 proteins are highly conserved and typically form a tetramer with histone H3. creating the nucleosome’s octameric core. Post-translational modifications (e.g., acetylation, methylation) on H4 residues play critical roles in epigenetic regulation. Recombinant human HIST2H4A protein is produced using expression systems like *E. coli* or yeast, ensuring high purity for experimental use. It serves as a tool for studying chromatin dynamics, histone-DNA interactions, and epigenetic mechanisms in diseases such as cancer, where aberrant histone modifications are common. Researchers also employ it to investigate synthetic nucleosome assembly, histone chaperone functions, and crosstalk between H4 modifications and other cellular pathways. Its recombinant form allows precise manipulation in biochemical assays, structural studies, and drug discovery targeting epigenetic regulators.
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