| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNT |
| Uniprot No | Q9P121 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MGVCGYLFLP WKCLVVVSLR LLFLVPTGVP VRSGDATFPK AMDNVTVRQG ESATLRCTID NRVTRVAWLN RSTILYAGND KWCLDPRVVL LSNTQTQYSI EIQNVDVYDE GPYTCSVQTD NHPKTSRVHL IVQVSPKIVE ISSDISINEG NNISLTCIAT GRPEPTVTWR HISPKAVGFV SEDEYLEIQG ITREQSGDYE CSASNDVAAP VVRRVKVTVN YPPYISEAKG TGVPVGQKGT LQCEASAVPS AEFQWYKDDK RLIEGKKGVK VENRPFLSKL IFFNVSEHDY GNYTCVASNK LGHTNASIML FG |
| 分子量 | 32 kDa |
| 蛋白标签 | His tag C-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的重组人HNT蛋白相关参考文献的模拟示例(注：文献为示例性虚构，实际引用时需检索真实数据库)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human HNT Protein in vitro*
**作者**：Smith, J. et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组人HNT蛋白，通过亲和层析纯化获得高纯度产物。实验验证了该蛋白在体外对神经细胞突触形成的促进作用，为HNT在神经再生领域的研究奠定了基础。
2. **文献名称**：*HNT Protein’s Role in Inflammatory Response: Insights from Recombinant Protein Delivery*
**作者**：Zhang, L. & Wang, H.
**摘要**：通过注射重组HNT蛋白至小鼠炎症模型，发现其显著抑制促炎因子TNF-α和IL-6的释放，提示HNT可能在免疫调节中具有潜在治疗价值。
3. **文献名称**：*Structural Analysis of Human HNT Protein and Its Interaction with DNA*
**作者**：Kuroda, M. et al.
**摘要**：采用X射线晶体学解析了重组HNT蛋白的三维结构，并证实其通过特定结构域与DNA损伤修复相关序列结合，为研究HNT在基因组稳定性中的作用提供机制依据。
4. **文献名称**：*Recombinant HNT Protein Enhances Wound Healing in Diabetic Rats*
**作者**：Gupta, R. et al.
**摘要**：实验证明，局部应用重组HNT蛋白可加速糖尿病大鼠皮肤创面的愈合过程，机制涉及促进血管生成和成纤维细胞迁移。
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**建议**：实际研究中，请通过PubMed、Web of Science等数据库检索真实文献，关键词可包括 **"recombinant human HNT protein"**、**"HNT protein function"** 或结合具体研究领域(如神经科学、炎症或结构生物学)。若HNT为特定领域简称，需明确其全称(如Hepatoma-Derived NT-related protein)以避免歧义。
**Background of Recombinant Human HNT Protein**
The HNT (Hematin) protein, encoded by the *HNT* gene, is a conserved eukaryotic protein implicated in cellular trafficking and membrane dynamics. Initially identified in *Drosophila* for its role in eye development and endocytic pathways, human HNT homologs share structural motifs, including a MARVEL domain associated with lipid bilayer interactions. Though its precise molecular mechanisms remain under investigation, HNT is suggested to regulate vesicle transport, lysosomal function, and receptor recycling, impacting processes like cell signaling, nutrient uptake, and tissue homeostasis.
Recombinant human HNT protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, bioactive forms for research and therapeutic exploration. Studies link HNT dysfunction to pathologies such as neurodegenerative disorders, cancer metastasis, and immune dysregulation, highlighting its potential as a biomarker or therapeutic target. Its recombinant form enables structural analysis, interaction studies, and screening for modulators in drug discovery. Current research focuses on elucidating HNT's role in autophagy, organelle crosstalk, and disease-specific pathways, aiming to leverage its regulatory functions for biomedical applications.
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