| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXD8 |
| Uniprot No | P13378 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-289aa |
| 氨基酸序列 | MSSYFVNPLYSKYKAAAAAAAAAGEAINPTYYDCHFAPGVGGRHAAAAAALQLYGNSAAGFPHAPPQAHAHPHPSPPPSGTGCGGREGRGQEYFHPGGGSPAAAYQAAPPPPPHPPPPPPPPPCGGIACHGEPAKFYGYDNLQRQPIFTTQQEAELVQYPDCKSSSGNIGEDPDHLNQSSSPSQMFPWMRPQAPGRRRGRQTYSRFQTLELEKEFLFNPYLTRKRRIEVSHALALTERQVKIWFQNRRMKWKKENNKDKFPVSRQEVKDGETKKEAQELEEDRAEGLTN |
| 分子量 | 58.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人HOXD8蛋白的研究文献及其摘要内容：
1. **文献名称**: *"Recombinant Human HOXD8 Protein Induces Osteogenic Differentiation in Mesenchymal Stem Cells via BMP Signaling"*
**作者**: Li, Y., et al.
**摘要**: 该研究报道了通过大肠杆菌系统表达并纯化的重组人HOXD8蛋白，并发现其能通过激活BMP/Smad通路促进间充质干细胞的成骨分化，揭示了HOXD8在骨再生中的潜在应用价值。
2. **文献名称**: *"Structural and Functional Analysis of HOXD8-DNA Interaction Using Recombinant Protein"*
**作者**: Smith, J.P., & Kumar, R.
**摘要**: 通过体外表达重组HOXD8蛋白，结合X射线晶体学技术解析其与靶DNA的结合模式，证实HOXD8通过特定的α螺旋结构域识别DNA序列，为理解HOX蛋白的基因调控机制提供结构基础。
3. **文献名称**: *"HOXD8 Recombinant Protein Suppresses Tumor Metastasis by Modulating EMT Markers in Breast Cancer Cells"*
**作者**: Wang, H., et al.
**摘要**: 研究使用哺乳动物细胞表达系统制备重组HOXD8蛋白，发现其能抑制乳腺癌细胞上皮间质转化(EMT)，降低侵袭能力，提示HOXD8可能作为癌症治疗的分子靶点。
4. **文献名称**: *"Efficient Expression and Purification of Recombinant HOXD8 for High-Throughput Screening Assays"*
**作者**: Gupta, S., et al.
**摘要**: 开发了一种基于昆虫细胞-杆状病毒系统的重组HOXD8蛋白高效表达和纯化方法，优化后的蛋白适用于高通量药物筛选，为HOX相关疾病的研究提供工具。
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**注**：上述文献及内容为示例性概括，具体研究请以实际发表的论文为准。若需真实文献，建议在PubMed或Web of Science中检索关键词“recombinant HOXD8 protein”。
HOXD8. a member of the HOX gene family, plays critical roles in embryonic development, particularly in patterning the anterior-posterior body axis and regulating cellular differentiation. As a transcription factor, it contains a conserved homeodomain that facilitates DNA binding to regulate target gene expression. Dysregulation of HOXD8 has been linked to developmental abnormalities and diseases, including certain cancers, due to its influence on cell proliferation and tissue morphogenesis.
Recombinant human HOXD8 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, followed by affinity purification to ensure high purity and biological activity. This recombinant form enables detailed studies of HOXD8's molecular mechanisms, including its DNA-binding specificity, interactions with co-regulatory proteins, and downstream signaling pathways. Researchers employ it in *in vitro* assays (e.g., electrophoretic mobility shift assays) and cellular models to dissect its role in developmental processes and disease pathogenesis. Additionally, it serves as a tool for screening therapeutic compounds targeting HOX-related disorders. By providing a standardized reagent, recombinant HOXD8 accelerates exploration of its dual functions in normal development and pathological contexts, bridging gaps between genetic studies and translational applications.
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