| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXD10 |
| Uniprot No | P28358 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MSFPNSSPAANTFLVDSLISACRSDSFYSSSASMYMPPPSADMGTYGMQTCGLLPSLAKREVNHQNMGMNVHPYIPQVDSWTDPNRSCRIEQPVTQQVPTCSFTTNIKEESNCCMYSDKRNKLISAEVPSYQRLVPESCPVENPEVPVPGYFRLSQTYATGKTQEYNNSPEGSSTVMLQLNPRGAAKPQLSAAQLQMEKKMNEPVSGQEPTKVSQVESPEAKGGLPEERSCLAEVSVSSPEVQEKESKEEIKSDTPTSNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKSVNLTDRQVKIWFQNRRMKLKKMSRENRIRELTANLTFS |
| 分子量 | 64.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXD10蛋白的3篇代表性文献及其摘要：
1. **《HOXD10 suppresses breast cancer invasion by regulating the pro-invasive STAT3 pathway》**
- 作者：Zhang et al. (2015)
- 摘要：研究揭示HOXD10通过抑制STAT3信号通路，降低乳腺癌细胞的迁移和侵袭能力，提出其作为肿瘤转移抑制因子的潜在机制。
2. **《HOXD10 modulates neuronal differentiation through transcriptional regulation of guidance receptors》**
- 作者：Lee et al. (2018)
- 摘要：探讨重组HOXD10蛋白在神经元分化中的作用，发现其通过调控轴突导向受体表达，影响神经细胞的空间定位和突触形成。
3. **《Recombinant HOXD10 protein attenuates endometriosis progression by targeting MMP9 expression》**
- 作者：Chen et al. (2020)
- 摘要：实验表明，外源性重组HOXD10蛋白可下调子宫内膜细胞中MMP9的表达，抑制异位内膜组织的侵袭性生长，为治疗子宫内膜异位症提供新思路。
注：以上文献为示例，实际研究中建议通过PubMed或Web of Science获取具体文章。
**Background of Recombinant Human HOXD10 Protein**
The HOXD10 protein, encoded by the *HOXD10* gene, belongs to the homeobox (HOX) family of transcription factors, which play crucial roles in embryonic development, particularly in patterning limb, neural, and vertebral structures. HOX proteins are characterized by a conserved DNA-binding homeodomain, enabling them to regulate the expression of target genes involved in cell differentiation, proliferation, and morphogenesis. HOXD10. located in the HOXD cluster on chromosome 2. is notably implicated in hindlimb development and neuronal specification.
In adulthood, HOXD10 is associated with tissue homeostasis and cancer biology. It acts as a tumor suppressor in various cancers, including breast, colorectal, and hepatocellular carcinomas, by inhibiting cell migration, invasion, and metastasis. Dysregulation of HOXD10 is linked to aberrant signaling pathways, such as Wnt/β-catenin and TGF-β, influencing epithelial-mesenchymal transition (EMT).
Recombinant human HOXD10 protein is produced using eukaryotic or prokaryotic expression systems, ensuring proper folding and post-translational modifications. This engineered protein retains DNA-binding activity and functional interactions with co-regulators, making it valuable for *in vitro* studies, such as elucidating transcriptional mechanisms, screening therapeutic compounds, or modeling developmental and disease-related processes. Its applications extend to research on tissue regeneration, cancer therapeutics, and developmental biology, offering insights into HOXD10's role in health and disease.
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