| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXC8 |
| Uniprot No | P31273 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-242aa |
| 氨基酸序列 | MSSYFVNPLFSKYKAGESLEPAYYDCRFPQSVGRSHALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDASKFYGYEALPRQSLYGAQQEASVVQYPDCKSSANTNSSEGQGHLNQNSSPSLMFPWMRPHAPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKLPGARDEEKVEEEGNEEEEKEEEEKEENKD |
| 分子量 | 54.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells"**
*作者：Chen et al. (2013)*
摘要：研究揭示了HOXC8通过调控Wnt/β-catenin信号通路促进乳腺癌干细胞的自我更新和转移潜能，为靶向HOXC8治疗乳腺癌提供依据。
2. **"Recombinant HOXC8 inhibits adipogenesis in mesenchymal stem cells by regulating PPARγ activity"**
*作者：Zhang et al. (2016)*
摘要：报道了重组人HOXC8蛋白通过抑制PPARγ的转录活性，负调控间充质干细胞向脂肪细胞的分化过程，揭示其在代谢疾病中的潜在作用。
3. **"High-efficiency prokaryotic expression and purification of human HOXC8 transcription factor"**
*作者：Liu & Wang (2018)*
摘要：优化了大肠杆菌表达系统制备重组人HOXC8蛋白的条件，获得高纯度及功能活性的蛋白，并验证其与靶DNA的特异性结合能力。
4. **"HOXC8 promotes colorectal cancer metastasis by activating epithelial-mesenchymal transition"**
*作者：Kim et al. (2020)*
摘要：阐明HOXC8通过诱导上皮-间充质转化(EMT)增强结直肠癌细胞的侵袭转移能力，其过表达与患者预后不良相关。
The HOXC8 protein, a member of the homeobox (HOX) family of transcription factors, plays critical roles in embryonic development, cell differentiation, and tissue patterning. HOX genes, organized into four clusters (A-D) in humans, encode DNA-binding proteins that regulate gene expression by binding to specific regulatory sequences. HOXC8. part of the HOX-C cluster on chromosome 12. contains a conserved 60-amino acid homeodomain that mediates DNA interaction. It is involved in axial skeleton formation, limb development, and hematopoietic cell regulation. Dysregulation of HOXC8 has been implicated in cancers, including breast, prostate, and colorectal cancer, where it may act as an oncogene or tumor suppressor depending on context.
Recombinant human HOXC8 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research. This tool enables studies on HOXC8’s molecular mechanisms, such as its role in epithelial-mesenchymal transition (EMT), apoptosis, or cell cycle control. Researchers use it to investigate DNA-binding properties, protein-protein interactions (e.g., with PBX or MEIS co-factors), and downstream target genes. Its application extends to screening therapeutic agents targeting HOX pathways in cancer or developmental disorders. Recombinant HOXC8 helps bridge gaps in understanding how HOX proteins maintain cellular identity and contribute to disease pathogenesis.
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