| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXC10 |
| Uniprot No | Q9NYD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKTINLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNFNFT |
| 分子量 | 37.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXC10蛋白的3条参考文献示例(文献信息为虚构示例，仅供参考格式)：
1. **《HOXC10蛋白在乳腺癌中的功能及机制研究》**
- **作者**：张伟等
- **摘要**：研究通过重组人HOXC10蛋白体外过表达实验，发现其能显著促进乳腺癌细胞的侵袭和迁移能力。机制研究表明，HOXC10通过激活Wnt/β-catenin信号通路诱导上皮-间充质转化(EMT)，进而促进肿瘤转移。
2. **《重组HOXC10蛋白对胃癌细胞增殖与凋亡的调控作用》**
- **作者**：李敏等
- **摘要**：本研究利用原核表达系统纯化重组HOXC10蛋白，并发现其通过抑制细胞周期相关蛋白(如Cyclin D1)表达，阻滞胃癌细胞G1/S期转换，同时诱导线粒体依赖性凋亡通路。
3. **《HOXC10重组蛋白的制备及其单克隆抗体的开发》**
- **作者**：王强等
- **摘要**：通过构建pET载体在大肠杆菌中高效表达重组HOXC10蛋白，经亲和层析纯化后免疫小鼠，成功制备出高特异性单克隆抗体，为HOXC10的临床检测提供工具。
(注：若需真实文献，建议通过PubMed、Google Scholar或Web of Science搜索关键词“recombinant human HOXC10 protein”获取。)
Recombinant human HOXC10 protein is a genetically engineered form of the HOXC10 protein, a member of the homeobox (HOX) gene family. HOX genes encode transcription factors critical for regulating embryonic development, particularly in establishing body patterning, cell differentiation, and tissue identity along the anterior-posterior axis. The HOXC10 protein contains a conserved 60-amino acid homeodomain, a DNA-binding motif that enables sequence-specific interactions with regulatory regions of target genes.
HOXC10 is primarily involved in limb development, nervous system organization, and urogenital tract formation. Studies suggest its role in cell proliferation, apoptosis, and migration. Dysregulation of HOXC10 has been implicated in pathological conditions, including cancers (e.g., breast, ovarian, and colorectal tumors), where it may act as either an oncogene or tumor suppressor depending on context. For example, elevated HOXC10 expression correlates with metastasis and poor prognosis in some malignancies but shows tumor-suppressive effects in others.
Recombinant HOXC10 is produced using prokaryotic or eukaryotic expression systems (e.g., E. coli, insect cells) for functional studies. It enables in vitro analysis of DNA-binding properties, protein-protein interactions, and downstream signaling pathways. Researchers utilize it to investigate developmental mechanisms, screen therapeutic compounds targeting HOXC10 activity, or standardize diagnostic assays. Tagged versions (e.g., His-tag, GST-tag) facilitate purification and detection. Its availability accelerates mechanistic insights into HOXC10's dual roles in development and disease.
×
