| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB6 |
| Uniprot No | P17509 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224aa |
| 氨基酸序列 | MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFATSSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEQPPFHPEPRKSDCAQDKSVFGETEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLELEKEFHYNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKESKLLSASQLSAEEEEEKQAE |
| 分子量 | 50.27 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《HOXB6在结肠癌细胞增殖与迁移中的作用机制研究》- 作者：Smith J.D. et al.**
摘要：该研究探讨了重组人HOXB6蛋白在结肠癌细胞中的功能，发现其过表达显著促进细胞增殖与迁移，可能通过调控Wnt/β-catenin信号通路发挥作用。
2. **《HOXB6重组蛋白在干细胞分化中的调控功能》- 作者：Chen L. et al.**
摘要：研究利用重组HOXB6蛋白处理小鼠胚胎干细胞，证实其通过激活特定转录因子(如SOX2)参与维持干细胞的未分化状态，并影响中胚层分化方向。
3. **《HOXB6异常表达与急性髓系白血病进展的关联》- 作者：Wang Y. et al.**
摘要：通过体外实验发现，重组HOXB6蛋白的过表达导致白血病细胞凋亡减少，并增强化疗耐药性，提示HOXB6可能作为AML治疗的潜在靶点。
4. **《HOXB6在胚胎肢体发育中的时空表达模式》- 作者：Garcia-Ruiz S. et al.**
摘要：研究利用重组HOXB6蛋白及基因编辑技术，揭示其在胚胎发育早期通过调控FGF信号通路，影响肢体骨骼模式的形成与细胞极性建立。
Homeobox B6 (HOXB6), a member of the homeobox gene family, encodes a transcription factor critical in embryonic development, particularly in limb patterning, hematopoiesis, and organogenesis. These genes, characterized by a conserved 60-amino acid DNA-binding homeodomain, regulate body axis formation and cell differentiation. HOXB6 is expressed in specific spatiotemporal patterns during embryogenesis, influencing progenitor cell fate and tissue morphogenesis. In adults, its dysregulation is implicated in cancers, including leukemia, breast cancer, and colorectal cancer, though its role remains controversial, acting as either an oncogene or tumor suppressor depending on context.
Recombinant human HOXB6 protein is engineered via heterologous expression systems (e.g., E. coli, mammalian cells) to study its molecular functions. Purified HOXB6 enables in vitro analyses of DNA-binding properties, transcriptional regulation, and interactions with cofactors like PBX and MEIS proteins. Researchers utilize it to explore mechanisms in developmental biology, cancer progression, and stem cell differentiation. Recent studies highlight its involvement in epithelial-mesenchymal transition (EMT) and metastasis, suggesting therapeutic targeting potential. However, challenges persist in resolving context-dependent signaling and post-translational modifications. Recombinant HOXB6 thus serves as a vital tool for dissecting its dual roles in development and disease.
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