| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXA6 |
| Uniprot No | P31267 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233aa |
| 氨基酸序列 | MSSYFVNPTFPGSLPSGQDSFLGQLPLYQAGYDALRPFPASYGASSLPDKTYTSPCFYQQSNSVLACNRASYEYGASCFYSDKDLSGASPSGSGKQRGPGDYLHFSPEQQYKPDSSSGQGKALHDEGADRKYTSPVYPWMQRMNSCAGAVYGSHGRRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIANALCLTERQIKIWFQNRRMKWKKENKLINSTQPSGEDSEAKAGE |
| 分子量 | 52.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXA6蛋白的3条示例参考文献(文献信息为虚构，仅用于示例)：
1. **文献名称**：*HOXA6蛋白在结肠癌中的异常表达及调控机制*
**作者**：Zhang Y, et al.
**摘要**：研究发现HOXA6通过抑制Wnt/β-catenin信号通路，调控结肠癌细胞增殖和迁移，重组HOXA6蛋白的体外过表达可抑制肿瘤生长。
2. **文献名称**：*重组人HOXA6蛋白在昆虫细胞中的表达与纯化*
**作者**：Lee S, et al.
**摘要**：利用杆状病毒表达系统成功制备重组HOXA6蛋白，通过质谱验证其结构，并证明该蛋白在体外能结合特定DNA序列调控靶基因表达。
3. **文献名称**：*HOXA6重组蛋白调控间充质干细胞成骨分化的作用*
**作者**：Gupta R, et al.
**摘要**：实验表明重组HOXA6可激活Runx2通路，促进间充质干细胞的成骨分化，提示其在治疗骨骼疾病中的潜在应用价值。
注：以上为示例性内容，实际文献需通过PubMed、Web of Science等数据库检索。
The human HOXA6 protein, a member of the HOX gene family, plays critical roles in embryonic development, particularly in establishing anterior-posterior body patterning and regulating cellular differentiation. HOX genes encode transcription factors characterized by a conserved DNA-binding homeodomain, enabling sequence-specific interactions with regulatory regions of target genes. HOXA6 is located within the HOXA cluster on chromosome 7 and is expressed in spatially restricted domains during embryogenesis, influencing tissue morphogenesis in regions such as the limb buds, nervous system, and hematopoietic lineages. Dysregulation of HOXA6 has been linked to developmental disorders and malignancies, including leukemia and solid tumors, highlighting its dual role in both normal development and oncogenesis.
Recombinant human HOXA6 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active forms for research. This engineered protein retains functional domains essential for DNA binding and protein-protein interactions, enabling studies on its regulatory mechanisms. Researchers utilize recombinant HOXA6 to investigate its transcriptional targets, epigenetic regulation, and cross-talk with signaling pathways like Wnt and TGF-β. Additionally, it serves as a tool for screening potential therapeutic agents targeting HOXA6-driven pathologies. Despite progress, functional redundancies with other HOX proteins and context-dependent roles in different tissues remain areas of active exploration. Its recombinant form thus provides a controlled platform to dissect HOXA6-specific contributions to development and disease.
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