| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXA10 |
| Uniprot No | P31260 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-393aa |
| 氨基酸序列 | MSCSESPAANSFLVDSLISSGRGEAGGGGGGAGGGGGGGYYAHGGVYLPPAADLPYGLQSCGLFPTLGGKRNEAASPGSGGGGGGLGPGAHGYGPSPIDLWLDAPRSCRMEPPDGPPPPPQQQPPPPPQPPQPAPQATSCSFAQNIKEESSYCLYDSADKCPKVSATAAELAPFPRGPPPDGCALGTSSGVPVPGYFRLSQAYGTAKGYGSGGGGAQQLGAGPFPAQPPGRGFDLPPALASGSADAARKERALDSPPPPTLACGSGGGSQGDEEAHASSSAAEELSPAPSESSKASPEKDSLGNSKGENAANWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISRSVHLTDRQVKIWFQNRRMKLKKMNRENRIRELTANFNFS |
| 分子量 | 69.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXA10蛋白的3篇代表性文献摘要信息：
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1. **标题**：HOXA10 regulates endometrial receptivity by controlling stromal cell proliferation and differentiation
**作者**：Taylor HS et al.
**摘要**：研究利用重组人HOXA10蛋白处理子宫内膜基质细胞，发现其通过激活整合素β3等分子增强胚胎着床能力，并调控细胞周期蛋白表达，提示HOXA10在优化子宫内膜容受性中的核心作用。
2. **标题**：Recombinant HOXA10 protein binds specific DNA motifs to regulate downstream gene networks
**作者**：Shi X et al.
**摘要**：通过大肠杆菌表达系统纯化重组人HOXA10蛋白，结合染色质免疫沉淀(ChIP)技术，揭示其直接结合胚胎发育相关基因的启动子区域(如Wnt通路基因)，调控细胞分化和器官形成。
3. **标题**：HOXA10 overexpression in leukemia cells alters hematopoietic differentiation
**作者**：Daga A et al.
**摘要**：实验通过向白血病细胞导入重组HOXA10蛋白，发现其异常表达干扰造血干细胞分化路径，导致增殖失控，为HOXA10在骨髓异常增生综合征中的作用提供机制依据。
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以上研究均涉及重组人HOXA10蛋白的应用，涵盖生殖医学、基因调控及血液疾病领域。如需扩展文献范围，建议进一步查阅近期关于HOXA10蛋白晶体结构或临床前模型的研究。
**Background of Recombinant Human HOXA10 Protein**
The HOXA10 gene, a member of the homeobox (HOX) gene family, encodes a transcription factor critical for embryonic development, cell differentiation, and tissue patterning. Specifically, HOXA10 regulates reproductive system development, uterine receptivity during implantation, and hematopoiesis. Dysregulation of HOXA10 expression is linked to endometriosis, recurrent pregnancy loss, leukemia, and other pathologies.
Recombinant human HOXA10 protein is synthesized in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce biologically active forms for functional studies. Its structure includes a conserved DNA-binding homeodomain, enabling sequence-specific interactions with target gene promoters to modulate transcription. Researchers employ recombinant HOXA10 to investigate its roles in cellular processes like proliferation, apoptosis, and epigenetic regulation.
In reproductive biology, HOXA10 governs endometrial stromal cell decidualization and embryo implantation timing. In hematopoiesis, it influences myeloid differentiation, with aberrant expression contributing to leukemia progression. Notably, HOXA10 exhibits dual oncogenic or tumor-suppressive roles depending on tissue context, highlighting its complex regulatory networks.
Studies using recombinant HOXA10 also explore post-translational modifications (e.g., phosphorylation, SUMOylation) that fine-tune its activity. As a research tool, it aids in drug discovery for fertility disorders or cancers. However, challenges remain in fully elucidating its context-dependent signaling and therapeutic potential. Overall, recombinant HOXA10 serves as a vital reagent for dissecting developmental and disease mechanisms linked to this multifaceted protein.
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