| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOOK2 |
| Uniprot No | Q96ED9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-717aa |
| 氨基酸序列 | MSVDKAELCGSLLTWLQTFHVPSPCASPQDLSSGLAVAYVLNQIDPSWFNEAWLQGISEDPGPNWKLKVSNLKMALRSLVEYSQDVLAHPVSEEHLPDVSLIGEFSDPAELGKLLQLVLGCAISCEKKQDHIQRIMTLEESVQHVVMEAIQELMTKDTPDSLSPETYGNFDSQSRRYYFLSEEAEEGDELQQRCLDLERQLMLLSEEKQSLAQENAGLRERMGRPEGEGTPGLTAKKLLLLQSQLEQLQEENFRLESGREDERLRCAELEREVAELQHRNQALTSLAQEAQALKDEMDELRQSSERAGQLEATLTSCRRRLGELRELRRQVRQLEERNAGHAERTRQLEDELRRAGSLRAQLEAQRRQVQELQGQRQEEAMKAEKWLFECRNLEEKYESVTKEKERLLAERDSLREANEELRCAQLQPRGLTQADPSLDPTSTPVDNLAAEILPAELRETLLRLQLENKRLCRQEAADRERQEELQRQLEDANRARHGLETQHRLNQQQLSELRAQVEDLQKALQEQGGKTEDSILLKRKLEEHLQKLHEADLELQRKREYIEELEPPTDSSTARRIEELRHNLQKKDADLRAMEERYRRYVDKARMVMQTMEPKQRPAAGAPPELHSLRTQLRERDVRIRHLEMDFEKSRSQREQEEKLLISAWYNMGMALQQRAGEERAPAHAQSFLAQQRLATNSRRGPLGRLASLNLRPTDKH |
| 分子量 | 104.39 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOOK2蛋白的3篇示例文献(内容综合现有研究概括，非真实文献，仅供参考)：
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1. **"Structural insights into human HOOK2 microtubule-binding domain and its role in cargo transport"**
*Zhang L. et al., Journal of Biological Chemistry (2018)*
摘要：通过重组表达人HOOK2蛋白的微管结合域(MBD)，解析其晶体结构并验证其与微管的相互作用，揭示HOOK2在细胞内囊泡运输中的调控机制。
2. **"HOOK2 regulates ciliogenesis through interaction with STAT3 signaling pathway"**
*Wang Y. et al., Molecular Biology of the Cell (2020)*
摘要：利用重组HOOK2蛋白进行体外互作实验，发现HOOK2通过结合STAT3调控初级纤毛形成，其功能缺失导致细胞周期紊乱，提示其在发育疾病中的潜在作用。
3. **"Recombinant HOOK2 inhibits hepatocellular carcinoma metastasis by suppressing EMT process"**
*Chen R. et al., Cancer Research (2021)*
摘要：研究重组HOOK2过表达对肝癌细胞的影响，发现其通过阻断EMT(上皮-间质转化)通路抑制肿瘤转移，为HOOK2的抑癌功能提供实验依据。
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**注**：以上为模拟内容，实际文献需通过PubMed或Google Scholar等平台检索，关键词如“recombinant human HOOK2”“HOOK2 protein function”等。
HOOK2. a member of the HOOK protein family, is a cytosolic adaptor protein implicated in microtubule-based endosomal trafficking and organelle positioning. It is evolutionarily conserved and structurally characterized by an N-terminal microtubule-binding domain, a central coiled-coil region mediating dimerization, and a C-terminal domain that interacts with cargo proteins or regulatory partners. In humans, HOOK2 localizes to the centrosome and surrounding structures, playing critical roles in maintaining Golgi apparatus integrity, regulating ciliogenesis, and facilitating vesicle transport. Its dysfunction has been linked to centriole duplication defects, ciliary disorders, and cancer progression.
Recombinant human HOOK2 protein is produced via heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, interactome, and cellular functions in vitro. Researchers utilize purified HOOK2 to investigate its microtubule-binding kinetics, phosphorylation regulation, and role in dynein-mediated transport. The recombinant form enables structure-function analysis through mutagenesis studies and serves as an antigen for antibody development. Current research focuses on HOOK2's involvement in cell cycle regulation, cancer cell motility, and neurological conditions, with emerging evidence suggesting potential therapeutic targets in HOOK2-associated pathways. Its interaction with Rab proteins and participation in autophagy-related processes further expand its relevance in cellular homeostasis studies.
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